Antisense targeting of TGF-beta1 augments BMP-induced upregulation of osteopontin, type I collagen and Cbfa1 in human Saos-2 cells.

Antisense targeting of TGF-beta1 augments BMP-induced upregulation of osteopontin, type I collagen and Cbfa1 in human Saos-2 cells. Research Abstract Details 

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Antisense targeting of TGF-beta1 augments BMP-induced upregulation of osteopontin, type I collagen and Cbfa1 in human Saos-2 cells. Abstract Text:

Zhong-Jian Shen,Sang Kook Kim,Do Youn Jun,Wan Park,Young Ho Kim,James S Malter,Byung Jo Moon,

Despite commonalities in signal transduction in osteoblasts from different species, the role of TGF-beta1 on bone formation remains elusive. In particular, the role of autocrine TGF-beta1 on human osteoblasts is largely unknown. Here we show the effect of TGF-beta1 knock-down on the proliferation and differentiation of osteoblasts induced by BMP2. Treatment with antisense TGF-beta1 moderately increased the rate of cell proliferation, which was completely reversed by the exogenous addition of TGF-beta1. Notably, TGF-beta1 blockade significantly enhanced BMP2-induced upregulation of mRNAs encoding osteopontin, type I collagen and Cbfa1, which was suppressed by exogenous TGF-beta1. Moreover, TGF-beta1 knock-down increased BMP2-induced phosphorylation of Smad1/5 as well as their nuclear import, which paralleled a reduction of inhibitory Smad6. These data suggest autocrine TGF-beta1 antagonizes BMP signaling through modulation of inducible Smad6 and the activity of BMP specific Smad1/5.

Antisense targeting of TGF-beta1 augments BMP-induced upregulation of osteopontin, type I collagen and Cbfa1 in human Saos-2 cells. Publishing Authors By Initials

ZJ Shen,SK Kim,DY Jun,W Park,YH Kim,JS Malter,BJ Moon,

For similar genetic processes: gene expression regulation: up-regulation research abstracts see: genetic processes: gene expression regulation: up-regulation research

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Antisense targeting of TGF-beta1 augments BMP-induced upregulation of osteopontin, type I collagen and Cbfa1 in human Saos-2 cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Experimental cell research

VOLUME: 313

Page Numbers: 1415-25

Journal Abbreviation:

ISSN: 0014-4827

DAY: 3

MONTH: 02

YEAR: 2007

Antisense targeting of TGF-beta1 augments BMP-induced upregulation of osteopontin, type I collagen and Cbfa1 in human Saos-2 cells. Information

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LANGUAGE: eng

NlmUniqueID: 373226

Antisense targeting of TGF-beta1 augments BMP-induced upregulation of osteopontin, type I collagen and Cbfa1 in human Saos-2 cells. Keywords Mesh Terms:

KEYWORDS: Up-Regulation

MESH TERMS: metabolism

Chemical & Substance for Abstract: Antisense targeting of TGF-beta1 augments BMP-induced upregulation of osteopontin, type I collagen and Cbfa1 in human Saos-2 cells. Information

Substance Name: phosphorodithioic acid

Registry Number: 15834-33-0

Grant and Affiliation Information for Antisense targeting of TGF-beta1 augments BMP-induced upregulation of osteopontin, type I collagen and Cbfa1 in human Saos-2 cells.

AFFILIATION: Department of Biochemistry, College of Natural Sciences, Kyungpook National University, Taegu 702-701, Korea. zshen2@wisc.edu <zshen2@wisc.edu>

Country: United States

AGENCY: United States NHLBI

GRANT: P50HL56396

ACRONYM: HL

MEDLINETA: Exp Cell Res

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