Antioxidative effect of Bacteroides thetaiotaomicron extracts: superoxide dismutase identification.

Antioxidative effect of Bacteroides thetaiotaomicron extracts: superoxide dismutase identification. Research Abstract Details 

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Antioxidative effect of Bacteroides thetaiotaomicron extracts: superoxide dismutase identification. Abstract Text:

Bacteroides thetaiotaomicron, a bowel anaerobic commensal, seems to release enzymes detoxifying reactive oxygen species according to our recent work. This opportunistic pathogen would be beneficial in the case of an inflammatory process. To explore its role after an oxidative or nutritive stress, six to seven separate experiments were performed. The bacteria were grown on media restricted in growth factors or supplemented with bile. Their viability was checked after surface protein extraction. The extracts underwent 2D electrophoresis. Gel images were statistically analysed to construct "master" gels. Proteins were identified (peptide-mass fingerprinting technique). The effect of each extract on superoxide anions was evaluated (spectrophotometric method). Superoxide dismutase was identified and a major superoxide anion inhibition was shown by extracts obtained after a nutritive and oxidative stress without significant bacterial death. So, a therapeutic antioxidant potential is firmly hoped for. [figure: see text]

Antioxidative effect of Bacteroides thetaiotaomicron extracts: superoxide dismutase identification. Publishing Authors By Initials

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Antioxidative effect of Bacteroides thetaiotaomicron extracts: superoxide dismutase identification. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Analytical and bioanalytical chemistry

VOLUME: 391

Page Numbers: 415-23

Journal Abbreviation:

ISSN: 1618-2650

DAY: 9

MONTH: 04

YEAR: 2008

Antioxidative effect of Bacteroides thetaiotaomicron extracts: superoxide dismutase identification. Information

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LANGUAGE: eng

NlmUniqueID: 101134327

Antioxidative effect of Bacteroides thetaiotaomicron extracts: superoxide dismutase identification. Keywords Mesh Terms:

KEYWORDS: Superoxides

MESH TERMS: chemistry

Chemical & Substance for Abstract: Antioxidative effect of Bacteroides thetaiotaomicron extracts: superoxide dismutase identification. Information

Substance Name: Superoxide Dismutase

Registry Number: EC 1.15.1.1

Grant and Affiliation Information for Antioxidative effect of Bacteroides thetaiotaomicron extracts: superoxide dismutase identification.

AFFILIATION: Laboratoire de Bactériologie Clinique, Faculté de Pharmacie de Lille, Université de Lille 2, 3 rue du Pr Laguesse, BP 83, 59006, Lille cedex, France.

Country: Germany

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MEDLINETA: Anal Bioanal Chem

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