Antigen presentation capacity of murine macrophages infected with Leishmania amazonensis amastigotes.

Antigen presentation capacity of murine macrophages infected with Leishmania amazonensis amastigotes. Research Abstract Details 

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Antigen presentation capacity of murine macrophages infected with Leishmania amazonensis amastigotes. Abstract Text:

Leishmania-infected M phi are potential candidates for the presentation of parasite Ag to Leishmania-specific CD4+ T lymphocytes. To assess whether infected cells could function as APC, we examined the ability of bone marrow-derived M phi infected with Leishmania amazonensis amastigotes to stimulate various CD4+, l-Ad- or l-Ed-restricted T-cell hybridomas specific for the bacteriophage lambda repressor cl protein, the human chorionic gonadotropin or OVA. A reduced capacity of infected M phi to present native Ag to most T-cell hybridomas tested was noted that was probably a result of a lower expression on their plasma membrane of stimulatory [la-peptide] complexes. Neither a reduced Ag uptake nor an altered Ag processing appeared to be at the origin of the partial inability of infected M phi to present Ag. As regards the level of plasma membrane la expression, no quantitative difference could be detected between uninfected and infected M phi. Moreover, after fixation with paraformaldehyde, the ability of plasma membrane la molecules to bind immunogenic peptides was apparently not reduced in infected M phi. So, these cells most likely expressed functional la molecules on their cell surface. Interestingly, infected M phi and M phi infected then cured by a treatment with a leishmanicidal compound were similarly impaired in their capacity to present native Ag or peptides to the hybridomas, and no recovery was noted even 24 h after the leishmanicidal treatment. Furthermore, infected M phi and M phi incubated with heat-killed amastigotes or with an amastigote homogenate exhibited similar inhibitions of Ag presentation. Taken together, these results suggest that the functional failure of infected M phi to present exogenous Ag could be because either of interferences with the events leading to the meeting of la molecules with peptides derived from these exogenous Ag or to a competition for binding to la molecules between these peptides and parasite molecules.

Antigen presentation capacity of murine macrophages infected with Leishmania amazonensis amastigotes. Publishing Authors By Initials

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Antigen presentation capacity of murine macrophages infected with Leishmania amazonensis amastigotes. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of immunology (Baltimore, Md. : 1950)

VOLUME: 151

Page Numbers: 2050-61

Journal Abbreviation: J. Immunol.

ISSN: 0022-1767

DAY: 15

MONTH: Aug

YEAR: 1993

Antigen presentation capacity of murine macrophages infected with Leishmania amazonensis amastigotes. Information

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LANGUAGE: eng

NlmUniqueID: 2985117

Antigen presentation capacity of murine macrophages infected with Leishmania amazonensis amastigotes. Keywords Mesh Terms:

KEYWORDS: Viral Regulatory and Accessory Proteins

MESH TERMS: immunology

Chemical & Substance for Abstract: Antigen presentation capacity of murine macrophages infected with Leishmania amazonensis amastigotes. Information

Substance Name: Ovalbumin

Registry Number: 9006-59-1

Grant and Affiliation Information for Antigen presentation capacity of murine macrophages infected with Leishmania amazonensis amastigotes.

AFFILIATION: Unité d'Immunophysiologie Cellulaire, Institut Pasteur, Paris, France.

Country: UNITED STATES

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MEDLINETA: J Immunol

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