Antifibrotic effects of interleukin-10 on experimental hepatic fibrosis.

Antifibrotic effects of interleukin-10 on experimental hepatic fibrosis. Research Abstract Details 

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Antifibrotic effects of interleukin-10 on experimental hepatic fibrosis. Abstract Text:

Li-Juan Zhang,Wei-Da Zheng,Yun-Xin Chen,Yue-Hong Huang,Zhi-Xin Chen,Sheng-Jun Zhang,Mei-Na Shi,Xiao-Zhong Wang,

BACKGROUND/AIMS: To study the effects of interleukin-10 on hepatic stellate cells and liver tissue in experimental rats hepatic fibrosis. METHODOLOGY: Rat hepatic fibrosis model induced by carbon tetrachloride was established. Liver tissues were harvested from the rats administered CCl4 with or without IL-10 treatment and the animals of the control group. The expression of TGF-beta1, MMP-2 and TIMP-1 in the liver tissues was measured by S-P immunohistochemistry. In addition, another model was established; HSCs in rats in each group were isolated. RT-PCR was employed to analyze TGF-beta1, MMP-2 and TIMP-1 mRNA expression in cells and immunocytochemistry was performed to detect protein expression of alpha-SMA, NF-kappaB, TGF-beta1, MMP-2 and TIMP-1 in HSCs. RESULTS: Rat hepatic fibrosis was developed successfully. The fibrosis changes were partially reversed by simultaneous administration of IL-10. The positive signals of TGF-beta1, MMP-2 and TIMP-1 were observed more frequently (P<0.05) in the CCl4-treated group compared to those in the IL-10-treated group and the control group. HSCs were successfully isolated. TGF-beta1, MMP-2 and TIMP-1 mRNA in HSCs increased obviously during the course of hepatic fibrosis, and their levels were decreased after the treatment with IL-10 (P<0.05). The immunocytochemistry positive levels for TGF-beta1, MMP-2, TIMP-1, alpha-SMA and NF-kappaB in the fibrogenesis group were increased significantly compared to the normal group (P<0.01). The positive signals decreased significantly (P<0.05) after the treatment with IL-10. CONCLUSIONS: The expression of TGF-beta1, MMP-2 and TIMP-1 increased in liver or in HSC of hepatic fibrosis rats and decreased after treatment with IL-10. The IL-10 could inhibit the activation of HSCs and make an antifibrogenic process come into effect in this way.

Antifibrotic effects of interleukin-10 on experimental hepatic fibrosis. Publishing Authors By Initials

LJ Zhang,WD Zheng,YX Chen,YH Huang,ZX Chen,SJ Zhang,MN Shi,XZ Wang,

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Antifibrotic effects of interleukin-10 on experimental hepatic fibrosis. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Hepato-gastroenterology

VOLUME: 54

Page Numbers: 2092-8

Journal Abbreviation: Hepatogastroenterology

ISSN: 0172-6390

DAY: 6

MONTH: 02

YEAR: 2008

Antifibrotic effects of interleukin-10 on experimental hepatic fibrosis. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Antifibrotic effects of interleukin-10 on experimental hepatic fibrosis.

AFFILIATION: Department of Gastroenterology, Union Hospital of Fujian Medical University, Fuzhou 350001, Fujian Province, China.

Country: Greece

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MEDLINETA: Hepatogastroenterology

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