Antibodies to trophoblast antigens HLA-G, placenta growth factor, and neuroD2 do not improve detection of circulating trophoblast cells in maternal blood.

Antibodies to trophoblast antigens HLA-G, placenta growth factor, and neuroD2 do not improve detection of circulating trophoblast cells in maternal blood. Research Abstract Details 

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Antibodies to trophoblast antigens HLA-G, placenta growth factor, and neuroD2 do not improve detection of circulating trophoblast cells in maternal blood. Abstract Text:

M L Tjoa,L Delli-Bovi,K L Johnson,D W Bianchi,

OBJECTIVES: Non-invasive prenatal diagnosis using circulating fetal trophoblast cells has been challenging due to lack of a reproducible trophoblast-specific antibody. We investigated the use of three trophoblast cell-specific antibodies, HLA-G, placenta growth factor, and neuroD2, for the isolation of trophoblast cells from the maternal circulation. METHODS: Trophoblast cells were isolated by density centrifugation from maternal blood samples (gestational age 10-20 weeks, n = 9). All women were carrying a male fetus. Following immunocytochemical staining with the trophoblast-specific antibodies, fluorescent in situ hybridization was performed, to verify whether any stained cells were indeed fetal. RESULTS: The HLA-G antibody had a ubiquitous staining pattern, which was not specific for trophoblast cells. Neither the placenta growth factor nor the neuroD2 antibodies were able to identify any trophoblast cells. Following fluorescent in situ hybridization, no male cells were detected on any of the slides. CONCLUSION: The antibodies used in this study were unable to improve detection of trophoblast cells in the maternal circulation.

Antibodies to trophoblast antigens HLA-G, placenta growth factor, and neuroD2 do not improve detection of circulating trophoblast cells in maternal blood. Publishing Authors By Initials

ML Tjoa,L Delli-Bovi,KL Johnson,DW Bianchi,

For similar cells: trophoblasts research abstracts see: cells: trophoblasts research

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Antibodies to trophoblast antigens HLA-G, placenta growth factor, and neuroD2 do not improve detection of circulating trophoblast cells in maternal blood. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Fetal diagnosis and therapy

VOLUME: 22

Page Numbers: 85-9

Journal Abbreviation: Fetal. Diagn. Ther.

ISSN: 1015-3837

DAY: 27

MONTH: 11

YEAR: 2006

Antibodies to trophoblast antigens HLA-G, placenta growth factor, and neuroD2 do not improve detection of circulating trophoblast cells in maternal blood. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9107463

Antibodies to trophoblast antigens HLA-G, placenta growth factor, and neuroD2 do not improve detection of circulating trophoblast cells in maternal blood. Keywords Mesh Terms:

KEYWORDS: Trophoblasts

MESH TERMS: immunology

Chemical & Substance for Abstract: Antibodies to trophoblast antigens HLA-G, placenta growth factor, and neuroD2 do not improve detection of circulating trophoblast cells in maternal blood. Information

Substance Name: product placenta growth factor

Registry Number: 144589-93-5

Grant and Affiliation Information for Antibodies to trophoblast antigens HLA-G, placenta growth factor, and neuroD2 do not improve detection of circulating trophoblast cells in maternal blood.

AFFILIATION: Division of Genetics, Department of Pediatrics, Tufts-New England Medical Center, Boston, MA 02111, USA.

Country: Switzerland

AGENCY: United States NIDDK

GRANT: P30 DK 34928

ACRONYM: DK

MEDLINETA: Fetal Diagn Ther

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