Anti-cancer activity of highly purified sulfur in immortalized and malignant human oral keratinocytes.

Anti-cancer activity of highly purified sulfur in immortalized and malignant human oral keratinocytes. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Anti-cancer activity of highly purified sulfur in immortalized and malignant human oral keratinocytes. Abstract Text:

Jun Lee,Hwa-Jeong Lee,Jong-Dae Park,Sun-Kyung Lee,Sang-Im Lee,Hyun-Dae Lim,You-Mee Lee,Young-Gab Yun,Byung-Hun Jeon,In-Soo Ree,Chang-Duk Jun,Suk-Keun Lee,Eun-Cheol Kim,

Sulfur is commonly used in Asia as an herbal medicine to treat inflammation and cancer, and potent chemopreventive effects have been demonstrated in various in vivo and in vitro models for sulfur-containing compounds found in naturally occurring products. Here, we report the growth inhibitory and apoptosis-related effects of a newly developed highly purified sulfur (HPS) on immortalized human oral keratinocytes (IHOKs) and on oral cancer cells representing two stages of oral cancer (HN4, HN12) based on a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, Western blotting, cell cycle analysis, and nuclear staining. The purity of the sulfur preparation was verified by high-performance liquid chromatography. HPS inhibited the proliferation of immortalized and malignant oral keratinocytes in a dose- and time-dependent manner. FITC-annexin V staining, DNA fragmentation testing, and Hoechst 33258 staining revealed that HPS inhibited cell growth via apoptosis. HPS increased the sub-G1 cell cycle fraction, with decreased expression of cyclins D1, D2, and E and their activating partners cdk2, cdk4, and cdk6, and a concomitant induction of p53 and p21/WAF1. Furthermore, HPS treatment increased the cytosolic level of cytochrome c and resulted in caspase-3 activation; this effect was correlated with Bax up-regulation and Bcl-2 down-regulation. Thus, these data suggest that HPS is a potential candidate for anti-cancer therapy in oral cancer.

Anti-cancer activity of highly purified sulfur in immortalized and malignant human oral keratinocytes. Publishing Authors By Initials

J Lee,HJ Lee,JD Park,SK Lee,SI Lee,HD Lim,YM Lee,YG Yun,BH Jeon,IS Ree,CD Jun,SK Lee,EC Kim,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Anti-cancer activity of highly purified sulfur in immortalized and malignant human oral keratinocytes. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Toxicology in vitro : an international journal pub

VOLUME: 22

Page Numbers: 87-95

Journal Abbreviation:

ISSN: 0887-2333

DAY: 1

MONTH: 09

YEAR: 2007

Anti-cancer activity of highly purified sulfur in immortalized and malignant human oral keratinocytes. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8712158

Anti-cancer activity of highly purified sulfur in immortalized and malignant human oral keratinocytes. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Anti-cancer activity of highly purified sulfur in immortalized and malignant human oral keratinocytes. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Anti-cancer activity of highly purified sulfur in immortalized and malignant human oral keratinocytes.

AFFILIATION: Department of Oral and Maxillofacial Surgery, College of Dentistry, Wonkwang University, Iksan, South Korea.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Toxicol In Vitro

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Anti-cancer activity of highly purified sulfur in immortalized and malignant human oral keratinocytes Related Publications

• Aberrant expression of MUC3 and MUC4 membrane-associated mucins and sialyl Le(x) antigen in pancreatic intraepithelial neoplasia.
• Aberrant expression of MUC5AC and MUC6 gastric mucins and sialyl Tn antigen in intraepithelial neoplasms of the pancreas.
• Advanced capabilities for future light sources.
• Aging of the population and the subsequent increase in the mistreatment of the elderly in Korea.
• Alkali-catalyzed beta-elimination of periodate-oxidized glycans: a novel method of chemical deglycosylation of mucin gene products in paraffin embedded sections.
• Altered mucin core peptide immunoreactivity in the colon polyp-carcinoma sequence.
• Basic fibroblast growth factor-induced translocation of p21-activated kinase to the membrane is independent of phospholipase C-gamma1 in the differentiation of PC12 cells.
• Biological properties and expression of mucins in 5-fluorouracil resistant HT29 human colon cancer cells.
• Cholangiocarcinomas arising in cirrhosis and combined hepatocellular-cholangiocellular carcinomas share apomucin profiles.
• Chromosomal clustering of muscle-expressed genes in Caenorhabditis elegans.
• Drynariae Rhizoma promotes osteoblast differentiation and mineralization in MC3T3-E1 cells through regulation of bone morphogenetic protein-2, alkaline phosphatase, type I collagen and collagenase-1.
• Elderly Women's Grief in Korea.
• Expression of MUC2, MUC5AC and MUC6 apomucins in carcinoma, dysplasia and non-dysplastic epithelia of the gallbladder.
• Goblet cell-specific expression mediated by the MUC2 mucin gene promoter in the intestine of transgenic mice.
• Identification and characterization of the MUC2 (human intestinal mucin) gene 5'-flanking region: promoter activity in cultured cells.
• Immunohistochemical study of mucin carbohydrates and core proteins in hepatolithiasis and cholangiocarcinoma.
• Induction of mucin gene expression in middle ear of rats by tumor necrosis factor-alpha: potential cause for mucoid otitis media.
• Initiation of transcription of the MUC3A human intestinal mucin from a TATA-less promoter and comparison with the MUC3B amino terminus.
• MUC3 human intestinal mucin. Analysis of gene structure, the carboxyl terminus, and a novel upstream repetitive region.
• Methylation status of promoters and expression of MUC2 and MUC5AC mucins in pancreatic cancer cells.
• Molecular cloning of human intestinal mucin (MUC2) cDNA. Identification of the amino terminus and overall sequence similarity to prepro-von Willebrand factor.
• Monoclonal antibodies against partially deglycosylated colon cancer mucin that recognize Tn antigen.
• Mouse intestinal goblet cells expressing SV40 T antigen directed by the MUC2 mucin gene promoter undergo apoptosis upon migration to the villi.
• Optical design of the U7 undulator beamline at the Pohang Light Source.
• Phorbol 12-myristate 13-acetate induces alteration in mucin gene expression and biological properties of colon cancer cells.
• Phorbol 12-myristate 13-acetate up-regulates the transcription of MUC2 intestinal mucin via Ras, ERK, and NF-kappa B.
• Stent-graft placement in a traumatic internal carotid-internal jugular fistula and pseudoaneurysm.
• Stereotactic aspiration of intracerebral haematoma: significance of surgical timing and haematoma volume reduction.
• Study of the aging perception.
• The Shank family of postsynaptic density proteins interacts with and promotes synaptic accumulation of the beta PIX guanine nucleotide exchange factor for Rac1 and Cdc42.