Anti-adenoviral effect of anti-HIV agents in vitro in serotypes inducing keratoconjunctivitis.

Anti-adenoviral effect of anti-HIV agents in vitro in serotypes inducing keratoconjunctivitis. Research Abstract Details 

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Anti-adenoviral effect of anti-HIV agents in vitro in serotypes inducing keratoconjunctivitis. Abstract Text:

Eiichi Uchio,Aki Fuchigami,Kazuaki Kadonosono,Akio Hayashi,Hiroaki Ishiko,Koki Aoki,Shigeaki Ohno,Eiichi Uchio,Aki Fuchigami,Kazuaki Kadonosono,Akio Hayashi,Hiroaki Ishiko,Koki Aoki,Shigeaki Ohno,

BACKGROUND: Around one million people are affected by adenoviral keratoconjunctivitis a year in Japan, and it is recognized as one of the major pathogens of ophthalmological nosocomial infection worldwide. Although cidofovir can be used systemically for immunocompromised patients with disseminated adenoviral infection, no specific anti-adenoviral agent has been established for the treatment of adenoviral infection. We evaluated the anti-adenoviral effect of anti-HIV (human immunodeficiency virus) agents in this study. METHODS: Five anti-HIV agents (zalcitabine, stavudine, nevirapine, indinavir and amprenavir) were subjected to in vitro evaluation. A549 cells were used for viral cell culture, and adenovirus serotypes 3, 4, 8, 19 and 37 were used. After calculating CC(50) (50% cytotoxic concentration) of each agent by MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) method, we cultured adenovirus with the agents for seven days and quantitatively measured extracted adenoviral DNA by real-time PCR. RESULTS: Among the anti-HIV drugs, zalcitabine and stavudine, both nucleoside reverse transcriptase inhibitors, showed significant anti-adenoviral activity. In contrast, nevirapine, a non-nucleoside reverse transcriptase inhibitor, and indinavir and amprenavir, which are both protease inhibitors, were ineffective against adenovirus. CONCLUSIONS: These results indicate that zalcitabine and stavudine are possible candidates for the local and systemic treatment of adenoviral infection, and the anti-adenoviral effect might depend on the pharmacological properties of anti-HIV agents. The chemical properties on the clinical safety for systemic and local application need to be determined in order to for these drugs to be accepted for the treatment of adenovirus in clinical settings.

Anti-adenoviral effect of anti-HIV agents in vitro in serotypes inducing keratoconjunctivitis. Publishing Authors By Initials

E Uchio,A Fuchigami,K Kadonosono,A Hayashi,H Ishiko,K Aoki,S Ohno,E Uchio,A Fuchigami,K Kadonosono,A Hayashi,H Ishiko,K Aoki,S Ohno,

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Anti-adenoviral effect of anti-HIV agents in vitro in serotypes inducing keratoconjunctivitis. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Graefe's archive for clinical and experimental oph

VOLUME: 245

Page Numbers: 1319-25

Journal Abbreviation: Graefes Arch. Clin. Exp. Ophth

ISSN: 0721-832X

DAY: 17

MONTH: 01

YEAR: 2007

Anti-adenoviral effect of anti-HIV agents in vitro in serotypes inducing keratoconjunctivitis. Information

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LANGUAGE: eng

NlmUniqueID: 8205248

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Grant and Affiliation Information for Anti-adenoviral effect of anti-HIV agents in vitro in serotypes inducing keratoconjunctivitis.

AFFILIATION: Department of Ophthalmology, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan-ku, Fukuoka 814-0180, Japan. euchio@fukuoka-u.ac.jp

Country: Germany

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MEDLINETA: Graefes Arch Clin Exp Ophthalm

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