Angiotensin II and its receptor subtypes in the human retina.

Angiotensin II and its receptor subtypes in the human retina. Research Abstract Details 

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Angiotensin II and its receptor subtypes in the human retina. Abstract Text:

Preenie deS Senanayake,Judy Drazba,Karen Shadrach,Amy Milsted,Elisabeth Rungger-Brandle,Kazutoshi Nishiyama,Shin-Ichiro Miura,Sadashiva Karnik,Jonathan E Sears,Joe G Hollyfield,

PURPOSE: To quantify and evaluate the distribution of angiotensin II (Ang II) and its receptors in the human retina. METHODS: Donor eyes were obtained within 12 hours postmortem and classified as hypertensive or normotensive and diabetic or nondiabetic, based on the donors' medical histories. Ang II in retina and vitreous was quantified by RIA. Ang II receptors were characterized and quantified by competitive membrane-binding assays. Ang II, its heptapeptide metabolite Ang-(1-7), and AT1 and AT2 receptors were localized by immunohistochemistry and confocal imaging. RESULTS: Levels of Ang II in the retina were significantly higher than in vitreous (P < 0.05). Ang II in the diabetic retina had a higher median compared with that in the nondiabetic retina. Ang II and Ang-(1-7) colocalized in retinal Müller cells. The retina had the highest levels of Ang II receptors that were significantly higher than the optic nerve, retinal pigment epithelium-choroid complex, and ciliary body-iris complex (P < 0.05). AT1 receptors were more abundant than AT2 receptors in the retina. Immunoreactivity for AT1 was detected in Müller cells and on blood vessels. AT2 receptors were localized throughout the Müller cells and nuclei of ganglion cells and neurons in the inner nuclear layer. CONCLUSIONS: In the human retina, identification of Ang II and its bioactive metabolite Ang-(1-7) in Müller cells suggests that these glial cells are able to produce and process Ang II. Ang receptors were localized in the blood vessels and neural cells. Local Ang II signaling may thus allow for autoregulation of neurovascular activity. Such an autonomous system could modulate the onset and severity of retinovascular disease.

Angiotensin II and its receptor subtypes in the human retina. Publishing Authors By Initials

P Senanayake,J Drazba,K Shadrach,A Milsted,E Rungger-Brandle,K Nishiyama,S Miura,S Karnik,JE Sears,JG Hollyfield,

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Angiotensin II and its receptor subtypes in the human retina. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Investigative ophthalmology & visual science

VOLUME: 48

Page Numbers: 3301-11

Journal Abbreviation: Invest. Ophthalmol. Vis. Sci.

ISSN: 0146-0404

DAY: 3

MONTH: Jul

YEAR: 2007

Angiotensin II and its receptor subtypes in the human retina. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7703701

Angiotensin II and its receptor subtypes in the human retina. Keywords Mesh Terms:

KEYWORDS: Tissue Donors

MESH TERMS: metabolism

Chemical & Substance for Abstract: Angiotensin II and its receptor subtypes in the human retina. Information

Substance Name: angiotensin converting enzyme 2

Registry Number: EC 3.4.17.-

Grant and Affiliation Information for Angiotensin II and its receptor subtypes in the human retina.

AFFILIATION: Department of Ophthalmology, Cole Eye Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA. senanap@ccf.org

Country: United States

AGENCY: United States NEI

GRANT: EY015638

ACRONYM: EY

MEDLINETA: Invest Ophthalmol Vis Sci

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