Angiogenic cells can be rapidly mobilized and efficiently harvested from the blood following treatment with AMD3100.

Angiogenic cells can be rapidly mobilized and efficiently harvested from the blood following treatment with AMD3100. Research Abstract Details 

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Angiogenic cells can be rapidly mobilized and efficiently harvested from the blood following treatment with AMD3100. Abstract Text:

Rebecca M Shepherd,Benjamin J Capoccia,Steven M Devine,John Dipersio,Kathryn M Trinkaus,David Ingram,Daniel C Link,

Circulating endothelial progenitor cells (EPCs) are thought to contribute to angiogenesis following vascular injury, stimulating interest in their ability to mediate therapeutic angiogenesis. However, the number of EPCs in the blood is low, limiting endogenous repair, and a method to rapidly mobilize EPCs has not been reported. In this study, healthy donors were mobilized sequentially with the CXCR4 antagonist, AMD3100, and G-CSF. The number of EPCs and circulating angiogenic cells (CACs) in the blood and pheresis product was determined and the angiogenic capacity of each cell population assessed. Compared with baseline, treatment with AMD3100 or G-CSF increased the number of blood CACs 10.0-fold +/- 4.4-fold and 8.8-fold +/- 3.7-fold, respectively. The number of EPCs in the blood increased 10.2-fold +/- 3.3-fold and 21.8-fold +/- 5.4-fold, respectively. On a percell basis, CACs harvested from G-CSF-mobilized blood displayed increased in vivo angiogenic potential compared with AMD3100-mobilized CACs. Mobilized EPCs displayed a greater proliferative capacity than EPCs isolated from baseline blood. Both CACs and EPCs were efficiently harvested by leukapheresis. Cryopreserved CACs but not EPCs retained functional activity after thawing. These data show that AMD3100 is a potent and rapid mobilizer of angiogenic cells and demonstrate the feasibility of obtaining and storing large numbers of angiogenic cells by leukapheresis.

Angiogenic cells can be rapidly mobilized and efficiently harvested from the blood following treatment with AMD3100. Publishing Authors By Initials

RM Shepherd,BJ Capoccia,SM Devine,J Dipersio,KM Trinkaus,D Ingram,DC Link,

For similar cells: stem cells research abstracts see: cells: stem cells research

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Angiogenic cells can be rapidly mobilized and efficiently harvested from the blood following treatment with AMD3100. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Blood

VOLUME: 108

Page Numbers: 3662-7

Journal Abbreviation: Blood

ISSN: 0006-4971

DAY: 15

MONTH: 08

YEAR: 2006

Angiogenic cells can be rapidly mobilized and efficiently harvested from the blood following treatment with AMD3100. Information

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LANGUAGE: eng

NlmUniqueID: 7603509

Angiogenic cells can be rapidly mobilized and efficiently harvested from the blood following treatment with AMD3100. Keywords Mesh Terms:

KEYWORDS: Stem Cells

MESH TERMS: physiology

Chemical & Substance for Abstract: Angiogenic cells can be rapidly mobilized and efficiently harvested from the blood following treatment with AMD3100. Information

Substance Name: JM 3100

Registry Number: 155148-31-5

Grant and Affiliation Information for Angiogenic cells can be rapidly mobilized and efficiently harvested from the blood following treatment with AMD3100.

AFFILIATION: Division of Oncology, Department of Medicine, 660 S Euclid Ave, Campus Box 8007, Saint Louis, MO 63110, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: T32 HL 07088-23

ACRONYM: HL

MEDLINETA: Blood

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