Angiogenic activity of bFGF and VEGF suppressed by proteolytic cleavage by neutrophil elastase.

Angiogenic activity of bFGF and VEGF suppressed by proteolytic cleavage by neutrophil elastase. Research Abstract Details 

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Angiogenic activity of bFGF and VEGF suppressed by proteolytic cleavage by neutrophil elastase. Abstract Text:

Shingo Ai,Xian Wu Cheng,Aiko Inoue,Kae Nakamura,Kenji Okumura,Akihisa Iguchi,Toyoaki Murohara,Masafumi Kuzuya,Shingo Ai,Xian Wu Cheng,Aiko Inoue,Kae Nakamura,Kenji Okumura,Akihisa Iguchi,Toyoaki Murohara,Masafumi Kuzuya,

Neutrophil elastase (NE), a serine protease released from the azurophil granules of activated neutrophil, proteolytically cleaves multiple cytokines, and cell surface proteins. In the present study, we examined whether NE affects the biological abilities of angiogenic growth factors such as basic-fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF). NE degraded bFGF and VEGF in a time- and concentration-dependent manner, and these degradations were suppressed by sivelestat, a synthetic inhibitor of NE. The bFGF- or VEGF-mediated proliferative activity of human umbilical vein endothelial cells was inhibited by NE, and the activity was recovered by sivelestat. Furthermore, NE reduced the bFGF- or VEGF-induced tubulogenic response of the mice aortas, ex vivo angiogenesis assay, and these effects were also recovered by sivelestat. Neutrophil-derived NE degraded potent angiogenic factors, resulting in loss of their angiogenic activity. These findings provide additional insight into the role played by neutrophils in the angiogenesis process at sites of inflammation.

Angiogenic activity of bFGF and VEGF suppressed by proteolytic cleavage by neutrophil elastase. Publishing Authors By Initials

S Ai,XW Cheng,A Inoue,K Nakamura,K Okumura,A Iguchi,T Murohara,M Kuzuya,S Ai,XW Cheng,A Inoue,K Nakamura,K Okumura,A Iguchi,T Murohara,M Kuzuya,

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Angiogenic activity of bFGF and VEGF suppressed by proteolytic cleavage by neutrophil elastase. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biochemical and biophysical research communication

VOLUME: 364

Page Numbers: 395-401

Journal Abbreviation: Biochem. Biophys. Res. Commun.

ISSN: 1090-2104

DAY: 15

MONTH: 10

YEAR: 2007

Angiogenic activity of bFGF and VEGF suppressed by proteolytic cleavage by neutrophil elastase. Information

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LANGUAGE: eng

NlmUniqueID: 372516

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Grant and Affiliation Information for Angiogenic activity of bFGF and VEGF suppressed by proteolytic cleavage by neutrophil elastase.

AFFILIATION: Department of Geriatrics, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.

Country: United States

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MEDLINETA: Biochem Biophys Res Commun

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