Androgen receptor regulation of the versican gene through an androgen response element in the proximal promoter.

Androgen receptor regulation of the versican gene through an androgen response element in the proximal promoter. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Androgen receptor regulation of the versican gene through an androgen response element in the proximal promoter. Abstract Text:

Jason T Read,Maziar Rahmani,Seti Boroomand,Sima Allahverdian,Bruce M McManus,Paul S Rennie,Jason T Read,Maziar Rahmani,Seti Boroomand,Sima Allahverdian,Bruce M McManus,Paul S Rennie,

Versican, one of the key components of prostatic stroma, plays a central role in tumor initiation and progression. Here, we investigated promoter elements and mechanisms of androgen receptor (AR)-mediated regulation of the versican gene in prostate cancer cells. Using transient transfection assays in prostate cancer LNCaP and cervical cancer HeLa cells engineered to express the AR, we demonstrate that the synthetic androgen R1881 and dihydrotestosterone stimulate expression of a versican promoter-driven luciferase reporter vector (versican-Luc). Further, both basal and androgen-stimulated versican-Luc activities were significantly diminished in LNCaP cells, when AR gene expression was knocked down using a short hairpin RNA. Methylation-protection footprinting analysis revealed an AR-protected element between positions +75 and +102 of the proximal versican promoter, which strongly resembled a consensus steroid receptor element. Electrophoretic mobility shift and supershift assays revealed strong and specific binding of the recombinant AR DNA binding domain to oligonucleotides corresponding to this protected DNA sequence. Site-directed mutagenesis of the steroid receptor element site markedly diminished R1881-stimulated versican-Luc activity. In contrast to the response seen using LNCaP cells, R1881 did not significantly induce versican promoter activity and mRNA levels in AR-positive prostate stromal fibroblasts. Interestingly, overexpression of beta-catenin in the presence of androgen augmented versican promoter activity 10- and 30-fold and enhanced versican mRNA levels 2.8-fold in fibroblasts. In conclusion, we demonstrate that AR transactivates versican expression, which may augment tumor-stromal interactions and may contribute to prostate cancer progression.

Androgen receptor regulation of the versican gene through an androgen response element in the proximal promoter. Publishing Authors By Initials

JT Read,M Rahmani,S Boroomand,S Allahverdian,BM McManus,PS Rennie,JT Read,M Rahmani,S Boroomand,S Allahverdian,BM McManus,PS Rennie,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Androgen receptor regulation of the versican gene through an androgen response element in the proximal promoter. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 31954-63

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 28

MONTH: 08

YEAR: 2007

Androgen receptor regulation of the versican gene through an androgen response element in the proximal promoter. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

Androgen receptor regulation of the versican gene through an androgen response element in the proximal promoter. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Androgen receptor regulation of the versican gene through an androgen response element in the proximal promoter. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Androgen receptor regulation of the versican gene through an androgen response element in the proximal promoter.

AFFILIATION: Prostate Center, Vancouver General Hospital, Department of Urologic Sciences, University of British Columbia, Vancouver, British Columbia V6T 2B5, Canada.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biol Chem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Androgen receptor regulation of the versican gene through an androgen response element in the proximal promoter Related Publications

• ACUTE RENAL CHANGES AFTER ORAL CHOLECYSTOGRAPHY.
• Adversity and the onset of psychiatric disorder in women.
• Androgen receptor regulation of the versican gene through an androgen response element in the proximal promoter.
• Computer literacy, skills and knowledge among dentists and professionals complementary to dentistry in Scotland.
• Cyclic post methotrexate dosing arthritis in systemic lupus erythematosus.
• Evidence for validity within workplace assessment: the Longitudinal Evaluation of Performance (LEP).
• Exercise- and nutrient-controlled mechanisms involved in maintenance of the musculoskeletal mass.
• Facets of protein metabolism in the clinical context: muscle RNA as an index of protein synthesis; and the problem of measuring the true rate of protein synthesis by identification of precursor labelling.
• Familial slipped upper femoral epiphysis.
• Gene expression responses over 24 h to lengthening and shortening contractions in human muscle: major changes in CSRP3, MUSTN1, SIX1, and FBXO32.
• How to get your abstract accepted for ESPEN.
• Is juvenile psychopathy associated with low anxiety and fear in conduct-disordered male offenders?
• Keynote-Anabolic Signals for Muscle Protein Metabolism: Effect of Food and Exercise: 459: 9:10 AM-9:35 AM.
• Management of symptomatic choroidal naevi with photodynamic therapy.
• Metabolic insights from the use of stable isotopes in nutritional studies.
• Metastasis-free survival following treatment for uveal melanoma with either stereotactic radiosurgery or enucleation.
• New approaches and recent results concerning human-tissue collagen synthesis.
• Outcome of bacteremia and fungemia in paediatric oncology patients.
• Prognosis in the psychoneuroses; benign and malignant developments.
• Psychiatric social work.
• Recent findings on the mechanism of glutamine stimulation of protein and carbohydrate anabolism.
• Sarcoma of the prostate treated with radiotherapy.
• So long, farewell.........
• The Structured Assessment of Violence Risk in Youth as a predictor of recidivism in a United Kingdom cohort of adolescent offenders with conduct disorder.
• The effects of photodynamic therapy on conjunctival in situ squamous cell carcinoma - a review of the histopathology.
• The inheritance of slipped upper femoral epiphysis.
• The pathology of slipped upper femoral epiphysis. A new concept.
• The temporal responses of protein synthesis, gene expression and cell signalling in human quadriceps muscle and patellar tendon to disuse.
• The urinary citrate excretion in patients with renal calculi.
• What do the JAMA editors say when they discuss manuscripts that they are considering for publication? Developing a schema for classifying the content of editorial discussion.