Anchorage-independent culture maintains prostate stem cells.

Anchorage-independent culture maintains prostate stem cells. Research Abstract Details 

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Anchorage-independent culture maintains prostate stem cells. Abstract Text:

Xudong Shi,Jerry Gipp,Wade Bushman,Xudong Shi,Jerry Gipp,Wade Bushman,Xudong Shi,Jerry Gipp,Wade Bushman,

Freshly isolated mouse prostate epithelial cells regenerate fully differentiated prostate tissue when combined with embryonic urogenital sinus mesenchyme and grafted in vivo. We show here that this regenerative capacity, which has been attributed to a small population of pleuripotential progenitor epithelial cells, is rapidly lost when the cells are placed in monolayer culture but can be maintained by culture in anchorage-independent conditions. Epithelial cells placed in anchorage-independent culture formed proliferating spheres that could be serially passaged and exhibited increased expression of putative stem cell markers as compared to cells grown in monolayer culture. Epithelial cells isolated from the fetal urogenital sinus, the newborn, and adult prostate formed spheres with similar efficiency, while cells isolated from the post-castration prostate exhibited significantly higher sphere-forming abilities. When passaged spheres were recombined with E17 rat urogenital sinus mesenchyme and grafted in vivo, they generated fully differentiated mouse prostate glandular epithelium containing both p63+ basal cells and p63- luminal cells and expressing a variety of prostate-specific and terminal differentiation markers.

Anchorage-independent culture maintains prostate stem cells. Publishing Authors By Initials

X Shi,J Gipp,W Bushman,X Shi,J Gipp,W Bushman,X Shi,J Gipp,W Bushman,

For similar cells: connective tissue cells: stromal cells research abstracts see: cells: connective tissue cells: stromal cells research

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Anchorage-independent culture maintains prostate stem cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Developmental biology

VOLUME: 312

Page Numbers: 396-406

Journal Abbreviation: Dev. Biol.

ISSN: 1095-564X

DAY: 29

MONTH: 09

YEAR: 2007

Anchorage-independent culture maintains prostate stem cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 372762

Anchorage-independent culture maintains prostate stem cells. Keywords Mesh Terms:

KEYWORDS: Stromal Cells

MESH TERMS: cytology

Chemical & Substance for Abstract: Anchorage-independent culture maintains prostate stem cells. Information

Substance Name: Bromodeoxyuridine

Registry Number: 59-14-3

Grant and Affiliation Information for Anchorage-independent culture maintains prostate stem cells.

AFFILIATION: Paul P. Carbone Comprehensive Cancer Center, University of Wisconsin-Madison, 600 Highland Avenue, Madison, Wisconsin 53792, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: R01 DK056238-05

ACRONYM: DK

MEDLINETA: Dev Biol

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