Analytical ultracentrifugation: sedimentation velocity and sedimentation equilibrium.

Analytical ultracentrifugation: sedimentation velocity and sedimentation equilibrium. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Analytical ultracentrifugation: sedimentation velocity and sedimentation equilibrium. Abstract Text:

James L Cole,Jeffrey W Lary,Thomas P Moody,Thomas M Laue,James L Cole,Jeffrey W Lary,Thomas P Moody,Thomas M Laue,

Analytical ultracentrifugation (AUC) is a versatile and powerful method for the quantitative analysis of macromolecules in solution. AUC has broad applications for the study of biomacromolecules in a wide range of solvents and over a wide range of solute concentrations. Three optical systems are available for the analytical ultracentrifuge (absorbance, interference, and fluorescence) that permit precise and selective observation of sedimentation in real time. In particular, the fluorescence system provides a new way to extend the scope of AUC to probe the behavior of biological molecules in complex mixtures and at high solute concentrations. In sedimentation velocity (SV), the movement of solutes in high centrifugal fields is interpreted using hydrodynamic theory to define the size, shape, and interactions of macromolecules. Sedimentation equilibrium (SE) is a thermodynamic method where equilibrium concentration gradients at lower centrifugal fields are analyzed to define molecule mass, assembly stoichiometry, association constants, and solution nonideality. Using specialized sample cells and modern analysis software, researchers can use SV to determine the homogeneity of a sample and define whether it undergoes concentration-dependent association reactions. Subsequently, more thorough model-dependent analysis of velocity and equilibrium experiments can provide a detailed picture of the nature of the species present in solution and their interactions.

Analytical ultracentrifugation: sedimentation velocity and sedimentation equilibrium. Publishing Authors By Initials

JL Cole,JW Lary,T P Moody,TM Laue,JL Cole,JW Lary,T P Moody,TM Laue,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Analytical ultracentrifugation: sedimentation velocity and sedimentation equilibrium. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Methods in cell biology

VOLUME: 84

Page Numbers: 143-79

Journal Abbreviation: Methods Cell Biol.

ISSN: 0091-679X

DAY: 29

MONTH: 10

YEAR: 2008

Analytical ultracentrifugation: sedimentation velocity and sedimentation equilibrium. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 373334

Analytical ultracentrifugation: sedimentation velocity and sedimentation equilibrium. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Analytical ultracentrifugation: sedimentation velocity and sedimentation equilibrium. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Analytical ultracentrifugation: sedimentation velocity and sedimentation equilibrium.

AFFILIATION: National Analytical Ultracentrifugation Facility, University of Connecticut, Storrs, Connecticut 06269, USA.

Country: United States

AGENCY: United States NCRR

GRANT: RR-18286

ACRONYM: RR

MEDLINETA: Methods Cell Biol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Analytical ultracentrifugation: sedimentation velocity and sedimentation equilibrium Related Publications

• Analytical ultracentrifugation: sedimentation velocity and sedimentation equilibrium.
• Diphtheria in Grays Harbor County, Washington.
• Immunolocalization of general odorant-binding protein in antennal sensilla of moth caterpillars.
• Structural Basis for the Recognition of Histone H4 by the Histone-Chaperone RbAp46.
• Structure determination of protein complexes by NMR.
• Structure of Cdc42 bound to the GTPase binding domain of PAK.
• [Aspects of healthy and pathologically changed laryngeal epithelium in a model. 2]
• [Healthy and pathologically changed laryngeal epithelium in a model. 1]
• [Involutional peculiarities of digestion and resorption--deduction of preventive and therapeutic needs]
• [Not Available.]
• [Relations between disordered exocrine pancreas function, alcohol consumption and age in alcoholics]
• [Senile changes in the resorption and elimination processes and their importance in pharmacotherapy]
• [The anthropology of guilt and guilt feelings]
• [To the self-organization of living systems as a concept of theoretical investigations in gerontology (author's transl)]