Analysis of tyrosine-o-sulfation.

Analysis of tyrosine-o-sulfation. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Analysis of tyrosine-o-sulfation. Abstract Text:

Jens R Bundgaard,Jette W Sen,Anders H Johnsen,Jens F Rehfeld,

Tyrosine O-sulfation was first described about 50 years ago as a post-translational modification of fibrinogen. In the following 30 years it was considered to be a rare modification affecting only a few proteins and peptides. However, in the beginning of the 1980s tyrosine (Tyr) sulfation was shown to be a common modification and since then an increasing number of proteins have been identified as sulfated. The target proteins belong to the classes of secretory, plasma membrane, and lysosomal proteins, which reflects the intracellular localization of the enzymes catalyzing Tyr sulfation, the tyrosylprotein sulfotransferases (TPSTs).Traditionally, Tyr sulfation has been analyzed by incorporation of radiolabeled sulfate into target cells followed by purification of the target protein. Subsequently, the protein is degraded enzymatically or by alkaline hydrolysis followed by thin-layer electrophoresis to demonstrate the presence of radioactively labeled tyrosine. These techniques have been described in detail previously. The aim of this chapter is to present alternative analytical methods of Tyr sulfation than radioisotope incorporation before analysis.

Analysis of tyrosine-o-sulfation. Publishing Authors By Initials

JR Bundgaard,JW Sen,AH Johnsen,JF Rehfeld,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Analysis of tyrosine-o-sulfation. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Methods in molecular biology (Clifton, N.J.)

VOLUME: 446

Page Numbers: 47-66

Journal Abbreviation:

ISSN: 1064-3745

DAY: 31

MONTH: 03

YEAR: 2008

Analysis of tyrosine-o-sulfation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9214969

Analysis of tyrosine-o-sulfation. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Analysis of tyrosine-o-sulfation. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Analysis of tyrosine-o-sulfation.

AFFILIATION:

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Methods Mol Biol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Analysis of tyrosine-o-sulfation Related Publications

• A chronic dermatosis of cattle due to oil applications.
• Analysis of tyrosine-o-sulfation.
• Distinct Linkage between Post-translational Processing and Differential Secretion of Progastrin Derivatives in Endocrine Cells.
• Gastrointestinal endocrinology.
• Handling of uncertainties for CE marking concerning Sound Transmission Loss of glazings.
• Insulin and glucose metabolism in liver cirrhosis and in liver failure.
• Letter: Gastrin heterogeneity: simple or complex?
• ProCNP and CNP are expressed primarily in male genital organs.
• Radioimmunoassay in diagnosis, localization and treatment of endocrine tumours in gut and pancreas.
• Radioimmunoassay of gastrin in human serum.
• Your unfair advantage.
• [Research activity in clinical biochemistry]