Analysis of transcription factor interactions in osteoblasts using competitive chromatin immunoprecipitation.

Analysis of transcription factor interactions in osteoblasts using competitive chromatin immunoprecipitation. Research Abstract Details 

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Analysis of transcription factor interactions in osteoblasts using competitive chromatin immunoprecipitation. Abstract Text:

Hernan Roca,Renny T Franceschi,

Chromatin immunoprecipitation (ChIP) is a widely used technique for quantifying protein-DNA interactions in living cells. This method commonly uses fixed (crosslinked) chromatin that is fragmented by sonication (X-ChIP). We developed a simple new ChIP procedure for the immunoprecipitation of sonicated chromatin isolated from osteoblasts in the absence of crosslinking (N-ChIP). The use of noncrosslinked chromatin allowed development of a new modification of the ChIP assay: the combination of N-ChIP and competition with double-stranded oligonucleotides containing specific binding sites for individual transcription factors (Competitive N-ChIP). Using this approach, we were able to discriminate between individual binding sites for the Runx2 transcription factor in the osteocalcin and bone sialoprotein genes that cannot be resolved by traditional X-ChIP. N-ChIP assays were also able to detect several other types of chromatin interactions including those with Dlx homeodomain factors and nuclear proteins such as Sin3a that lack an intrinsic DNA-binding motif and, therefore, bind to chromatin via interactions with other proteins.

Analysis of transcription factor interactions in osteoblasts using competitive chromatin immunoprecipitation. Publishing Authors By Initials

H Roca,RT Franceschi,

For similar proteins: transcription factors research abstracts see: proteins: transcription factors research

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Analysis of transcription factor interactions in osteoblasts using competitive chromatin immunoprecipitation. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Nucleic acids research

VOLUME: 36

Page Numbers: 1723-30

Journal Abbreviation: Nucleic Acids Res.

ISSN: 1362-4962

DAY: 7

MONTH: 02

YEAR: 2008

Analysis of transcription factor interactions in osteoblasts using competitive chromatin immunoprecipitation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 411011

Analysis of transcription factor interactions in osteoblasts using competitive chromatin immunoprecipitation. Keywords Mesh Terms:

KEYWORDS: Transcription Factors

MESH TERMS: metabolism

Chemical & Substance for Abstract: Analysis of transcription factor interactions in osteoblasts using competitive chromatin immunoprecipitation. Information

Substance Name: Osteocalcin

Registry Number: 104982-03-8

Grant and Affiliation Information for Analysis of transcription factor interactions in osteoblasts using competitive chromatin immunoprecipitation.

AFFILIATION: Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, MI, USA.

Country: England

AGENCY: United States NIDCR

GRANT: DE11723

ACRONYM: DE

MEDLINETA: Nucleic Acids Res

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