Analysis of transcription factor HY5 genomic binding sites revealed its hierarchical role in light regulation of development.

Analysis of transcription factor HY5 genomic binding sites revealed its hierarchical role in light regulation of development. Research Abstract Details 

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Analysis of transcription factor HY5 genomic binding sites revealed its hierarchical role in light regulation of development. Abstract Text:

Jungeun Lee,Kun He,Viktor Stolc,Horim Lee,Pablo Figueroa,Ying Gao,Waraporn Tongprasit,Hongyu Zhao,Ilha Lee,Xing Wang Deng,

The transcription factor LONG HYPOCOTYL5 (HY5) acts downstream of multiple families of the photoreceptors and promotes photomorphogenesis. Although it is well accepted that HY5 acts to regulate target gene expression, in vivo binding of HY5 to any of its target gene promoters has yet to be demonstrated. Here, we used a chromatin immunoprecipitation procedure to verify suspected in vivo HY5 binding sites. We demonstrated that in vivo association of HY5 with promoter targets is not altered under distinct light qualities or during light-to-dark transition. Coupled with DNA chip hybridization using a high-density 60-nucleotide oligomer microarray that contains one probe for every 500 nucleotides over the entire Arabidopsis thaliana genome, we mapped genome-wide in vivo HY5 binding sites. This analysis showed that HY5 binds preferentially to promoter regions in vivo and revealed >3000 chromosomal sites as putative HY5 binding targets. HY5 binding targets tend to be enriched in the early light-responsive genes and transcription factor genes. Our data thus support a model in which HY5 is a high hierarchical regulator of the transcriptional cascades for photomorphogenesis.

Analysis of transcription factor HY5 genomic binding sites revealed its hierarchical role in light regulation of development. Publishing Authors By Initials

J Lee,K He,V Stolc,H Lee,P Figueroa,Y Gao,W Tongprasit,H Zhao,I Lee,XW Deng,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: protein binding research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: protein binding research

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Analysis of transcription factor HY5 genomic binding sites revealed its hierarchical role in light regulation of development. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Plant cell

VOLUME: 19

Page Numbers: 731-49

Journal Abbreviation:

ISSN: 1040-4651

DAY: 2

MONTH: 03

YEAR: 2007

Analysis of transcription factor HY5 genomic binding sites revealed its hierarchical role in light regulation of development. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9208688

Analysis of transcription factor HY5 genomic binding sites revealed its hierarchical role in light regulation of development. Keywords Mesh Terms:

KEYWORDS: Protein Binding

MESH TERMS: radiation effects

Chemical & Substance for Abstract: Analysis of transcription factor HY5 genomic binding sites revealed its hierarchical role in light regulation of development. Information

Substance Name: flavanone synthetase

Registry Number: EC 2.3.1.74

Grant and Affiliation Information for Analysis of transcription factor HY5 genomic binding sites revealed its hierarchical role in light regulation of development.

AFFILIATION: Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Conecticut 06520-8104, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM-47850

ACRONYM: GM

MEDLINETA: Plant Cell

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