Analysis of the extracellular matrix vesicle proteome in mineralizing osteoblasts.

Analysis of the extracellular matrix vesicle proteome in mineralizing osteoblasts. Research Abstract Details 

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Analysis of the extracellular matrix vesicle proteome in mineralizing osteoblasts. Abstract Text:

Zhen Xiao,Corinne E Camalier,Kunio Nagashima,King C Chan,David A Lucas,M Jason de la Cruz,Michelle Gignac,Stephen Lockett,Haleem J Issaq,Timothy D Veenstra,Thomas P Conrads,George R Beck,

Many key processes central to bone formation and homeostasis require the involvement of osteoblasts, cells responsible for accumulation and mineralization of the extracellular matrix (ECM). During this complex and only partially understood process, osteoblasts generate and secrete matrix vesicles (MVs) into the ECM to initiate mineralization. Although they are considered an important component of mineralization process, MVs still remain a mystery. To better understand their function and biogenesis, a proteomic analysis of MVs has been conducted. MVs were harvested by two sample preparation approaches and mass spectrometry was utilized for protein identification. A total of 133 proteins were identified in common from the two MV preparations, among which were previously known proteins, such as annexins and peptidases, along with many novel proteins including a variety of enzymes, osteoblast-specific factors, ion channels, and signal transduction molecules, such as 14-3-3 family members and Rab-related proteins. To compare the proteome of MV with that of the ECM we conducted a large-scale proteomic analysis of collagenase digested mineralizing osteoblast matrix. This analysis resulted in the identification of 1,327 unique proteins. A comparison of the proteins identified from the two MV preparations with the ECM analysis revealed 83 unique, non-redundant proteins identified in all three samples. This investigation represents the first systematic proteomic analysis of MVs and provides insights into both the function and origin of these important mineralization-regulating vesicles.

Analysis of the extracellular matrix vesicle proteome in mineralizing osteoblasts. Publishing Authors By Initials

Z Xiao,CE Camalier,K Nagashima,KC Chan,DA Lucas,MJ de la Cruz,M Gignac,S Lockett,HJ Issaq,TD Veenstra,TP Conrads,GR Beck,

For similar biological sciences: biochemistry: proteomics research abstracts see: biological sciences: biochemistry: proteomics research

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Analysis of the extracellular matrix vesicle proteome in mineralizing osteoblasts. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of cellular physiology

VOLUME: 210

Page Numbers: 325-35

Journal Abbreviation: J. Cell. Physiol.

ISSN: 0021-9541

DAY: 3

MONTH: Feb

YEAR: 2007

Analysis of the extracellular matrix vesicle proteome in mineralizing osteoblasts. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 50222

Analysis of the extracellular matrix vesicle proteome in mineralizing osteoblasts. Keywords Mesh Terms:

KEYWORDS: Proteomics

MESH TERMS: metabolism

Chemical & Substance for Abstract: Analysis of the extracellular matrix vesicle proteome in mineralizing osteoblasts. Information

Substance Name: Proteome

Registry Number: 0

Grant and Affiliation Information for Analysis of the extracellular matrix vesicle proteome in mineralizing osteoblasts.

AFFILIATION: Laboratory of Proteomics and Analytical Technologies, SAIC-Frederick, Inc., NCI-Frederick, Frederick, Maryland 21702, USA.

Country: United States

AGENCY: United States NCI

GRANT: N01-CO12400

ACRONYM: CO

MEDLINETA: J Cell Physiol

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