Analysis of the essential DNA region for OsEBP-89 promoter in response to methyl jasmonic acid.

Analysis of the essential DNA region for OsEBP-89 promoter in response to methyl jasmonic acid. Research Abstract Details 

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Analysis of the essential DNA region for OsEBP-89 promoter in response to methyl jasmonic acid. Abstract Text:

Ang Li,Liangliang Chen,Haiyun Ren,Xuechen Wang,Haiwen Zhang,Rong-Feng Huang,

In rice, the characterization of OsEBP-89 is inducible by various stress-or hormone-stimuli, including ethylene, abscisic acid (ABA), jasmonate acid (JA), drought and cold. Here, we report the investigation of essential DNA region within OsEBP-89 promoter for methyl jasmonic acid (MeJA) induction. PLACE analysis indicates that this promoter sequence contains multiple potential elements in response to various stimuli. First, we fused this promoter with GUS gene and analyzed its expression under MeJA treatment through Agrobacterium infiltration mediating transient expression in tobacco leaves. Our results revealed that this chimeric gene could be inducible by MeJA in tobacco leaves. To further determine the crucial sequences responsible for MeJA induction, we generated a series of deletion promoters which were fused with GUS reporter gene respectively. The results of transient expression of GUS gene driven by these mutant promoters show that the essential region for MeJA induction is positioned in the region between -1200 and -800 in OsEBP-89 promoter containing a G-box (-1127), which is distinct from the essential region containing ERE (-562) for ACC induction. In all, our finding is helpful in understanding the molecular mechanism of OsEBP-89 expression under different stimuli. OsEBP-89, essential DNA region, methyl jasmonic acid, transient assay, promoter, tobacco leaves.

Analysis of the essential DNA region for OsEBP-89 promoter in response to methyl jasmonic acid. Publishing Authors By Initials

A Li,L Chen,H Ren,X Wang,H Zhang,RF Huang,

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Analysis of the essential DNA region for OsEBP-89 promoter in response to methyl jasmonic acid. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Science in China. Series C, Life sciences / Chines

VOLUME: 51

Page Numbers: 280-5

Journal Abbreviation: Sci. China, C, Life Sci.

ISSN: 1006-9305

DAY: 4

MONTH: Mar

YEAR: 2008

Analysis of the essential DNA region for OsEBP-89 promoter in response to methyl jasmonic acid. Information

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LANGUAGE: eng

NlmUniqueID: 9611809

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Grant and Affiliation Information for Analysis of the essential DNA region for OsEBP-89 promoter in response to methyl jasmonic acid.

AFFILIATION: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

Country: China

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MEDLINETA: Sci China C Life Sci

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