Analysis of the C. elegans Argonaute family reveals that distinct Argonautes act sequentially during RNAi.

Analysis of the C. elegans Argonaute family reveals that distinct Argonautes act sequentially during RNAi. Research Abstract Details 

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Analysis of the C. elegans Argonaute family reveals that distinct Argonautes act sequentially during RNAi. Abstract Text:

Argonaute (AGO) proteins interact with small RNAs to mediate gene silencing. C. elegans contains 27 AGO genes, raising the question of what roles these genes play in RNAi and related gene-silencing pathways. Here we describe 31 deletion alleles representing all of the previously uncharacterized AGO genes. Analysis of single- and multiple-AGO mutant strains reveals functions in several pathways, including (1) chromosome segregation, (2) fertility, and (3) at least two separate steps in the RNAi pathway. We show that RDE-1 interacts with trigger-derived sense and antisense RNAs to initiate RNAi, while several other AGO proteins interact with amplified siRNAs to mediate downstream silencing. Overexpression of downstream AGOs enhances silencing, suggesting that these proteins are limiting for RNAi. Interestingly, these AGO proteins lack key residues required for mRNA cleavage. Our findings support a two-step model for RNAi, in which functionally and structurally distinct AGOs act sequentially to direct gene silencing.

Analysis of the C. elegans Argonaute family reveals that distinct Argonautes act sequentially during RNAi. Publishing Authors By Initials

For similar proteins: recombinant proteins: recombinant fusion proteins research abstracts see: proteins: recombinant proteins: recombinant fusion proteins research

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Analysis of the C. elegans Argonaute family reveals that distinct Argonautes act sequentially during RNAi. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Cell

VOLUME: 127

Page Numbers: 747-57

Journal Abbreviation: Cell

ISSN: 0092-8674

DAY: 17

MONTH: Nov

YEAR: 2006

Analysis of the C. elegans Argonaute family reveals that distinct Argonautes act sequentially during RNAi. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 413066

Analysis of the C. elegans Argonaute family reveals that distinct Argonautes act sequentially during RNAi. Keywords Mesh Terms:

KEYWORDS: Recombinant Fusion Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Analysis of the C. elegans Argonaute family reveals that distinct Argonautes act sequentially during RNAi. Information

Substance Name: rde-1 protein, C elegans

Registry Number: 0

Grant and Affiliation Information for Analysis of the C. elegans Argonaute family reveals that distinct Argonautes act sequentially during RNAi.

AFFILIATION: Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM58800

ACRONYM: GM

MEDLINETA: Cell

REFSOURCE: Cell. 2006 Nov 17;127(4):667-8

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