Analysis of tear glucose concentration with electrospray ionization mass spectrometry.

Analysis of tear glucose concentration with electrospray ionization mass spectrometry. Research Abstract Details 

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Analysis of tear glucose concentration with electrospray ionization mass spectrometry. Abstract Text:

Christopher R Taormina,Justin T Baca,Sanford A Asher,Joseph J Grabowski,David N Finegold,Christopher R Taormina,Justin T Baca,Sanford A Asher,Joseph J Grabowski,David N Finegold,

We have developed a mass spectrometry-based method that allows one to accurately determine the glucose concentration of tear fluid. We used a 1 microL micro-capillary to collect tear fluid from the tear meniscus with minimal irritation of the eye. We analyzed the 1 muL volume of collected tear fluid with liquid-chromatography electrospray ionization mass spectrometry with the use of D-glucose-6,6-d2 as an internal standard. Repeated measurements and a recovery experiment on pooled, onion-induced tears showed that the analysis of the glucose in tears was precise (4% relative standard deviation) and provided 100% recovery. We found the tear glucose concentration of one fasting nondiabetic subject to be 13 to 51 microM while the onion-induced tear glucose concentration of a different nondiabetic subject to be 211 to 256 microM.

Analysis of tear glucose concentration with electrospray ionization mass spectrometry. Publishing Authors By Initials

CR Taormina,JT Baca,SA Asher,JJ Grabowski,DN Finegold,CR Taormina,JT Baca,SA Asher,JJ Grabowski,DN Finegold,

For similar fluids and secretions: bodily secretions: tears research abstracts see: fluids and secretions: bodily secretions: tears research

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Analysis of tear glucose concentration with electrospray ionization mass spectrometry. Journal Published:

PUBLICATION TYPE: Validation Studies

Journal: Journal of the American Society for Mass Spectrome

VOLUME: 18

Page Numbers: 332-6

Journal Abbreviation: J. Am. Soc. Mass Spectrom.

ISSN: 1044-0305

DAY: 2

MONTH: 11

YEAR: 2006

Analysis of tear glucose concentration with electrospray ionization mass spectrometry. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9010412

Analysis of tear glucose concentration with electrospray ionization mass spectrometry. Keywords Mesh Terms:

KEYWORDS: Tears

MESH TERMS: metabolism

Chemical & Substance for Abstract: Analysis of tear glucose concentration with electrospray ionization mass spectrometry. Information

Substance Name: Glucose

Registry Number: 50-99-7

Grant and Affiliation Information for Analysis of tear glucose concentration with electrospray ionization mass spectrometry.

AFFILIATION: Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: DK-55348

ACRONYM: DK

MEDLINETA: J Am Soc Mass Spectrom

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