Analysis of RNA export using Xenopus oocytes.

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Analysis of RNA export using Xenopus oocytes. Abstract Text:

D Duricka,K S Ullman,D Duricka,K S Ullman,

This unit describes a procedure for monitoring RNA export in Xenopus oocytes. The technique involves synthesizing labeled RNA in vitro and microinjecting the RNA into oocyte nuclei. Following incubation the oocytes are dissected into nuclear and cytoplasmic fractions. These samples are then processed for RNA analysis, allowing the extent of export to be quantitatively assessed.

Analysis of RNA export using Xenopus oocytes. Publishing Authors By Initials

D Duricka,KS Ullman,D Duricka,KS Ullman,

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Analysis of RNA export using Xenopus oocytes. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Current protocols in cell biology / editorial boar

VOLUME: Chapter 11

Page Numbers: Unit 11.14

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ISSN: 1934-2616

DAY: 29

MONTH: May

YEAR: 2001

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LANGUAGE: eng

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AFFILIATION: University of Utah, Salt Lake City, Utah, USA.

Country: United States

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MEDLINETA: Curr Protoc Cell Biol

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