Analysis of phosphorylation of unlabeled proteins.

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Analysis of phosphorylation of unlabeled proteins. Abstract Text:

B M Sefton,B M Sefton,

Phosphorylation of unlabeled proteins can be detected using immunological or enzymatic techniques. Anti-phosphotyrosine antibodies are used with immunoblots to detect tyrosine phosphorylation. This unit provides a protocol for detection of antibodies by (125)I-labeled protein A or enhanced chemiluminescence (ECL). Detection of enzymatic dephosphorylation using a general or phosphoamino acid-specific phosphatase and subsequent SDS-PAGE mobility shift (or a change in activity) is also described.

Analysis of phosphorylation of unlabeled proteins. Publishing Authors By Initials

BM Sefton,BM Sefton,

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Analysis of phosphorylation of unlabeled proteins. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Current protocols in molecular biology / edited by

VOLUME: Chapter 18

Page Numbers: Unit18.4

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ISSN: 1934-3647

DAY: 11

MONTH: May

YEAR: 2001

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LANGUAGE: eng

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AFFILIATION: The Salk Institute, San Diego, California.

Country: United States

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MEDLINETA: Curr Protoc Mol Biol

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