Analysis of phospho- and phosphonosphingolipids by high-performance liquid chromatography.

Analysis of phospho- and phosphonosphingolipids by high-performance liquid chromatography. Research Abstract Details 

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Analysis of phospho- and phosphonosphingolipids by high-performance liquid chromatography. Abstract Text:

O Itasaka,T Hori,K Sasahara,Y Wakabayashi,F Takahashi,H Rhee,

A simple and efficient method for the separation of phosphosphingolipids including phosphonosphingolipids by high-performance liquid chromatography is described. A mixture of authentic lipids consisting of sphingomyelin, ceramide phosphorylethanolamine, ceramide 2-aminoethylphosphonate, and ceramide N-methylaminoethylphosphonate was completely separated using a silica gel (Zorbax SIL) column with acetonitrile-methanol-water 72:40:10 (v/v) as eluting solvent. The elution of these sphingolipids was monitored directly with an ultraviolet spectromonitor at 207 nm. The practical limit of detection of each sphingolipid was about 0.2 microgram or 0.3 nmol. Using this method, we found that from one to four different phosphono- and/or phosphosphingolipids in fresh-water shellfish can be routinely identified and reproducibly quantified.

Analysis of phospho- and phosphonosphingolipids by high-performance liquid chromatography. Publishing Authors By Initials

O Itasaka,T Hori,K Sasahara,Y Wakabayashi,F Takahashi,H Rhee,

For similar lipids: membrane lipids: sphingolipids research abstracts see: lipids: membrane lipids: sphingolipids research

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Analysis of phospho- and phosphonosphingolipids by high-performance liquid chromatography. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 95

Page Numbers: 1671-5

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jun

YEAR: 1984

Analysis of phospho- and phosphonosphingolipids by high-performance liquid chromatography. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Analysis of phospho- and phosphonosphingolipids by high-performance liquid chromatography. Keywords Mesh Terms:

KEYWORDS: Sphingolipids

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: Analysis of phospho- and phosphonosphingolipids by high-performance liquid chromatography. Information

Substance Name: phosphonolipids

Registry Number: 0

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Country: JAPAN

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MEDLINETA: J Biochem

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