Analysis of P element transposase protein-DNA interactions during the early stages of transposition.

Analysis of P element transposase protein-DNA interactions during the early stages of transposition. Research Abstract Details 

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Analysis of P element transposase protein-DNA interactions during the early stages of transposition. Abstract Text:

Mei Tang,Ciro Cecconi,Carlos Bustamante,Donald C Rio,

P elements are a family of transposable elements found in Drosophila that move by using a cut-and-paste mechanism and that encode a transposase protein that uses GTP as a cofactor for transposition. Here we used atomic force microscopy to visualize the initial interaction of transposase protein with P element DNA. The transposase first binds to one of the two P element ends, in the presence or absence of GTP, prior to synapsis. In the absence of GTP, these complexes remain stable but do not proceed to synapsis. In the presence of GTP or nonhydrolyzable GTP analogs, synapsis happens rapidly, whereas DNA cleavage is slow. Both atomic force microscopy and standard biochemical methods have been used to show that the P element transposase exists as a pre-formed tetramer that initially binds to either one of the two P element ends in the absence of GTP prior to synapsis. This initial single end binding may explain some of the aberrant P element-induced rearrangements observed in vivo, such as hybrid end insertion. The allosteric effect of GTP in promoting synapsis by P element transposase may be to orient a second site-specific DNA binding domain in the tetramer allowing recognition of a second high affinity transposase-binding site at the other transposon end.

Analysis of P element transposase protein-DNA interactions during the early stages of transposition. Publishing Authors By Initials

M Tang,C Cecconi,C Bustamante,DC Rio,

For similar enzymes and coenzymes: enzymes: recombinases: integrases: transposases research abstracts see: enzymes and coenzymes: enzymes: recombinases: integrases: transposases research

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Analysis of P element transposase protein-DNA interactions during the early stages of transposition. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 29002-12

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 19

MONTH: 07

YEAR: 2007

Analysis of P element transposase protein-DNA interactions during the early stages of transposition. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

Analysis of P element transposase protein-DNA interactions during the early stages of transposition. Keywords Mesh Terms:

KEYWORDS: Transposases

MESH TERMS: metabolism

Chemical & Substance for Abstract: Analysis of P element transposase protein-DNA interactions during the early stages of transposition. Information

Substance Name: Transposases

Registry Number: EC 2.7.7.-

Grant and Affiliation Information for Analysis of P element transposase protein-DNA interactions during the early stages of transposition.

AFFILIATION: Department of Molecular and Cell Biology, Division of Genetics, Genomics and Development, Center for Integrative Genomics, University of California, Berkeley, California 94720, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: R01GM61987

ACRONYM: GM

MEDLINETA: J Biol Chem

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