Analysis of P-body assembly in Saccharomyces cerevisiae.

Analysis of P-body assembly in Saccharomyces cerevisiae. Research Abstract Details 

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Analysis of P-body assembly in Saccharomyces cerevisiae. Abstract Text:

Daniela Teixeira,Roy Parker,

Recent experiments have defined cytoplasmic foci, referred to as processing bodies (P-bodies), that contain untranslating mRNAs in conjunction with proteins involved in translation repression and mRNA decapping and degradation. However, the order of protein assembly into P-bodies and the interactions that promote P-body assembly are unknown. To gain insight into how yeast P-bodies assemble, we examined the P-body accumulation of Dcp1p, Dcp2p, Edc3p, Dhh1p, Pat1p, Lsm1p, Xrn1p, Ccr4p, and Pop2p in deletion mutants lacking one or more P-body component. These experiments revealed that Dcp2p and Pat1p are required for recruitment of Dcp1p and of the Lsm1-7p complex to P-bodies, respectively. We also demonstrate that P-body assembly is redundant and no single known component of P-bodies is required for P-body assembly, although both Dcp2p and Pat1p contribute to P-body assembly. In addition, our results indicate that Pat1p can be a nuclear-cytoplasmic shuttling protein and acts early in P-body assembly. In contrast, the Lsm1-7p complex appears to primarily function in a rate limiting step after P-body assembly in triggering decapping. Taken together, these results provide insight both into the function of individual proteins involved in mRNA degradation and the mechanisms by which yeast P-bodies assemble.

Analysis of P-body assembly in Saccharomyces cerevisiae. Publishing Authors By Initials

D Teixeira,R Parker,

For similar proteins: fungal proteins: saccharomyces cerevisiae proteins research abstracts see: proteins: fungal proteins: saccharomyces cerevisiae proteins research

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MEDLINE DATE:

Analysis of P-body assembly in Saccharomyces cerevisiae. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Molecular biology of the cell

VOLUME: 18

Page Numbers: 2274-87

Journal Abbreviation: Mol. Biol. Cell

ISSN: 1059-1524

DAY: 11

MONTH: 04

YEAR: 2007

Analysis of P-body assembly in Saccharomyces cerevisiae. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9201390

Analysis of P-body assembly in Saccharomyces cerevisiae. Keywords Mesh Terms:

KEYWORDS: Saccharomyces cerevisiae Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Analysis of P-body assembly in Saccharomyces cerevisiae. Information

Substance Name: DHH1 protein, S cerevisiae

Registry Number: EC 3.6.1.-

Grant and Affiliation Information for Analysis of P-body assembly in Saccharomyces cerevisiae.

AFFILIATION: Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721-0106, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: R37 GM45443

ACRONYM: GM

MEDLINETA: Mol Biol Cell

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