Analysis of N-glycan in serum glycoproteins from db/db mice and humans with type 2 diabetes.

Analysis of N-glycan in serum glycoproteins from db/db mice and humans with type 2 diabetes. Research Abstract Details 

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Analysis of N-glycan in serum glycoproteins from db/db mice and humans with type 2 diabetes. Abstract Text:

Naofumi Itoh,Shinji Sakaue,Hiroaki Nakagawa,Masaki Kurogochi,Hiroshi Ohira,Kisaburo Deguchi,Shin-Ichiro Nishimura,Masaharu Nishimura,Naofumi Itoh,Shinji Sakaue,Hiroaki Nakagawa,Masaki Kurogochi,Hiroshi Ohira,Kisaburo Deguchi,Shin-Ichiro Nishimura,Masaharu Nishimura,

Glycosylation has an important role in regulating properties of proteins and is associated with many diseases. To examine the alteration of serum N-glycans in type 2 diabetes, we used the db/db mouse model. Serum N-glycans were fluorescence labeled and applied to HPLC. There were reproducible differences in N-glycan profiles between the db/db mouse model and the db/+ control. The structures of the oligosaccharides, which had changed in their amounts, were analyzed by a two-dimensional mapping method, matrix-assisted laser desorption ionization-time-of-flight mass spectrometry, and exoglycosidase digestion. Those analyses revealed an increase in the N-glycans possessing alpha1,6-fucose in the serum of db/db mice. The level of alpha1,6-fucosyltransferase mRNA was increased in the liver of the db/db mice. The ratio of a biantennary N-glycan with alpha1,6-fucose to that without alpha1,6-fucose in the liver tissue of the db/db mouse was increased relative to the db/+ control. Next, we analyzed the serum N-glycan profile in human subjects with type 2 diabetes and found an increased amount of a biantennary N-glycan that had an alpha1,6-fucose with a bisecting N-acetylglucosamine. In conclusion, the increase in alpha1,6-fucosylation is a striking change in the serum N-glycans of the db/db mice, whereas the change in the fucosylation in humans with type 2 diabetes was small, albeit statistically significant. It is likely that the change is caused, at least partially, by the increase in the alpha1,6-fucosyltransferase mRNA level in the liver. The increased alpha1,6-fucosylation may affect protein properties associated with the pathophysiology of type 2 diabetes.

Analysis of N-glycan in serum glycoproteins from db/db mice and humans with type 2 diabetes. Publishing Authors By Initials

N Itoh,S Sakaue,H Nakagawa,M Kurogochi,H Ohira,K Deguchi,S Nishimura,M Nishimura,N Itoh,S Sakaue,H Nakagawa,M Kurogochi,H Ohira,K Deguchi,S Nishimura,M Nishimura,

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Analysis of N-glycan in serum glycoproteins from db/db mice and humans with type 2 diabetes. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: American journal of physiology. Endocrinology and

VOLUME: 293

Page Numbers: E1069-77

Journal Abbreviation: Am. J. Physiol. Endocrinol. Me

ISSN: 0193-1849

DAY: 31

MONTH: 07

YEAR: 2007

Analysis of N-glycan in serum glycoproteins from db/db mice and humans with type 2 diabetes. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Analysis of N-glycan in serum glycoproteins from db/db mice and humans with type 2 diabetes.

AFFILIATION: First Dept. of Medicine, Graduate School of Medicine, Hokkaido University, Sapporo 060-8638, Japan.

Country: United States

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MEDLINETA: Am J Physiol Endocrinol Metab

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