Analysis of Mycoplasma hyorhinis genome by use of restriction endonucleases and by electron microscopy.

Analysis of Mycoplasma hyorhinis genome by use of restriction endonucleases and by electron microscopy. Research Abstract Details 

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Analysis of Mycoplasma hyorhinis genome by use of restriction endonucleases and by electron microscopy. Abstract Text:

The chromosome of Mycoplasma hyorhinis was analyzed by using different restriction endonucleases and electron microscopy. It was found that restriction enzymes BstEII, XhoI, and SacI are the enzymes of choice for analysis and characterization of M. hyorhinis. The bands resulting from digestion of M. hyorhinis DNA with BstEII had apparent molecular weights ranging from 1.2 X 10(6) to 75 X 10(6). The apparent total molecular weight of DNA was calculated from the molecular weights of the individual bands and found to be 251 X 10(6). Electron microscopic contour length measurements of the largest DNA fragments verified the molecular weight values calculated from gel analysis. Electron microscopic contour length measurements of intact DNA of M. hyorhinis revealed a molecular weight of 5.4 +/- 5 X 10(8). The discrepancy between the values of molecular weight of M. hyorhinis DNA as determined by restriction enzyme analysis and contour length measurement is based on the fact that some of the DNA fragments which migrate as an apparent single band in the agarose gel really are double or multiple DNA fragments.

Analysis of Mycoplasma hyorhinis genome by use of restriction endonucleases and by electron microscopy. Publishing Authors By Initials

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Analysis of Mycoplasma hyorhinis genome by use of restriction endonucleases and by electron microscopy. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of bacteriology

VOLUME: 150

Page Numbers: 788-94

Journal Abbreviation: J. Bacteriol.

ISSN: 0021-9193

DAY: 22

MONTH: May

YEAR: 1982

Analysis of Mycoplasma hyorhinis genome by use of restriction endonucleases and by electron microscopy. Information

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LANGUAGE: eng

NlmUniqueID: 2985120

Analysis of Mycoplasma hyorhinis genome by use of restriction endonucleases and by electron microscopy. Keywords Mesh Terms:

KEYWORDS: Mycoplasma

MESH TERMS: genetics

Chemical & Substance for Abstract: Analysis of Mycoplasma hyorhinis genome by use of restriction endonucleases and by electron microscopy. Information

Substance Name: GGTNACC-specific type II deoxyribonuclea

Registry Number: EC 3.1.21.4

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Country: UNITED STATES

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MEDLINETA: J Bacteriol

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