Analysis of mitogen-activated protein kinase activation and interactions with regulators and substrates.

Analysis of mitogen-activated protein kinase activation and interactions with regulators and substrates. Research Abstract Details 

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Analysis of mitogen-activated protein kinase activation and interactions with regulators and substrates. Abstract Text:

Lee Bardwell,Kandarp Shah,

Mitogen-activated protein kinase (MAPK) cascades are ubiquitous signal transduction modules in eukaryotes that are of great interest and importance. Here, we summarize some useful methods for the analysis of MAPK signaling, including methods to (1) detect MAPK activation in cells, with an emphasis on using phosphorylation-state-specific antibodies raised against mammalian phosphopeptide sequences to detect the activation of MAPKs in other species; (2) estimate the cellular concentrations of MAPKs and other proteins of interest; (3) detect and quantify the stable physical association of MAPKs with their substrates and regulators, and estimate the relevant dissociation constants; (4) delineate the MAPK-binding regions or domains of MAPK-interacting proteins, with particular emphasis on the identification and verification of MAPK-docking sites. These procedures are broadly applicable to many organisms, including both yeast and mammalian cells.

Analysis of mitogen-activated protein kinase activation and interactions with regulators and substrates. Publishing Authors By Initials

L Bardwell,K Shah,

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Analysis of mitogen-activated protein kinase activation and interactions with regulators and substrates. Journal Published:

PUBLICATION TYPE: Review

Journal: Methods (San Diego, Calif.)

VOLUME: 40

Page Numbers: 213-23

Journal Abbreviation: Methods

ISSN: 1046-2023

DAY: 3

MONTH: Nov

YEAR: 2006

Analysis of mitogen-activated protein kinase activation and interactions with regulators and substrates. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9426302

Analysis of mitogen-activated protein kinase activation and interactions with regulators and substrates. Keywords Mesh Terms:

KEYWORDS: Proteins

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: Analysis of mitogen-activated protein kinase activation and interactions with regulators and substrates. Information

Substance Name: Mitogen-Activated Protein Kinases

Registry Number: EC 2.7.1.37

Grant and Affiliation Information for Analysis of mitogen-activated protein kinase activation and interactions with regulators and substrates.

AFFILIATION: Department of Developmental and Cell Biology, University of California, Irvine, CA 92697, USA. bardwell@uci.edu

Country: United States

AGENCY: United States NIGMS

GRANT: GM69013

ACRONYM: GM

MEDLINETA: Methods

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