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Analysis of membrane proteins of Mycoplasma capricolum strains by SDS-PAGE and immunoblotting.

Analysis of membrane proteins of Mycoplasma capricolum strains by SDS-PAGE and immunoblotting. Research Abstract Details 

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  • Analysis of membrane proteins of Mycoplasma capricolum strains by SDS-PAGE and immunoblotting. Abstract Text:

    a benkiraneA Benkirane,s amgharS Amghar,h kirchhoffH Kirchhoff,

    Sixteen moroccan and five other Mycoplasma (M.) capricolum strains were characterized by SDS-PAGE as well as by the immunobinding assay and immunoblotting, using antisera against M. capricolum California Kid (CK) and M. mycoides YG. There was a strong reaction of all 21 strains with antiserum against M. capricolum CK and of 18 strains with antiserum against M. mycoides YG in the immunobinding assay, confirming the cross-reactivity between these two species observed earlier. A marked heterogeneity among the M. capricolum strains appeared in SDS-PAGE and in immunoblotting, characterized by different protein patterns and different strengths of identical protein bands. All M. capricolum strains investigated revealed, however, at least three strong protein bands with mol. weights of about 30, 42 and 52 KD reacting in immunoblotting with antiserum against M. capricolum CK but not with antiserum against M. mycoides YG. These proteins may represent antigens suitable for the identification and differentiation of M. capricolum strains.

    Analysis of membrane proteins of Mycoplasma capricolum strains by SDS-PAGE and immunoblotting. Publishing Authors By Initials

    a benkiraneA Benkirane,s amgharS Amghar,h kirchhoffH Kirchhoff,

    For similar animal diseases: sheep diseases research abstracts see: animal diseases: sheep diseases research

    PUBMED ID PMID:

    MEDLINE DATE:

    Analysis of membrane proteins of Mycoplasma capricolum strains by SDS-PAGE and immunoblotting. Journal Published:

    PUBLICATION TYPE: Research Support, Non-U.S. Gov

    Journal: Zentralblatt für Veterinärmedizin. Reihe B. Journa

    VOLUME: 40

    Page Numbers: 119-24

    Journal Abbreviation: Zentralblatt Veterinarmedizin

    ISSN: 0514-7166

    DAY: 13

    MONTH: Mar

    YEAR: 1993

    Analysis of membrane proteins of Mycoplasma capricolum strains by SDS-PAGE and immunoblotting. Information

    Number of References:

    LANGUAGE: eng

    NlmUniqueID: 331325

    Analysis of membrane proteins of Mycoplasma capricolum strains by SDS-PAGE and immunoblotting. Keywords Mesh Terms:

    KEYWORDS: Sheep Diseases

    MESH TERMS: microbiology

    Chemical & Substance for Abstract: Analysis of membrane proteins of Mycoplasma capricolum strains by SDS-PAGE and immunoblotting. Information

    Substance Name: Membrane Proteins

    Registry Number: 0

    Grant and Affiliation Information for Analysis of membrane proteins of Mycoplasma capricolum strains by SDS-PAGE and immunoblotting.

    AFFILIATION: Departement de Microbiologie, Immunologie et Maladies contagieuses, Institut Agronomique et Vétérinaire, Rabat, Maroc.

    Country: GERMANY

    GERMANY Research PublicationGERMANY Research Publication

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    GRANT:

    ACRONYM:

    MEDLINETA: Zentralbl Veterinarmed B

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