Analysis of genetic interactions on a genome-wide scale in budding yeast: diploid-based synthetic lethality analysis by microarray.

Analysis of genetic interactions on a genome-wide scale in budding yeast: diploid-based synthetic lethality analysis by microarray. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Analysis of genetic interactions on a genome-wide scale in budding yeast: diploid-based synthetic lethality analysis by microarray. Abstract Text:

Pamela B Meluh,Xuewen Pan,Daniel S Yuan,Carol Tiffany,Ou Chen,Sharon Sookhai-Mahadeo,Xiaoling Wang,Brian D Peyser,Rafael Irizarry,Forrest A Spencer,Jef D Boeke,

Comprehensive collections of open reading frame (ORF) deletion mutant strains exist for the budding yeast Saccharomyces cerevisiae. With great prescience, these strains were designed with short molecular bar codes or TAGs that uniquely mark each deletion allele, flanked by shared priming sequences. These features have enabled researchers to handle yeast mutant collections as complex pools of approximately 6000 strains. The presence of any individual mutant within a pool can be assessed indirectly by measuring the relative abundance of its corresponding TAG(s) in genomic DNA prepared from the pool. This is readily accomplished by wholesale polymerase chain reaction (PCR) amplification of the TAGs using fluorescent oligonucleotide primers that recognize the common flanking sequences, followed by hybridization of the labeled PCR products to a TAG oligonucleotide microarray. Here we describe a method-diploid-based synthetic lethality analysis by microarray (dSLAM)-whereby such pools can be manipulated to rapidly construct and assess the fitness of 6000 double-mutant strains in a single experiment. Analysis of double-mutant strains is of growing importance in defining the spectrum of essential cellular functionalities and in understanding how these functionalities interrelate.

Analysis of genetic interactions on a genome-wide scale in budding yeast: diploid-based synthetic lethality analysis by microarray. Publishing Authors By Initials

PB Meluh,X Pan,DS Yuan,C Tiffany,O Chen,S Sookhai-Mahadeo,X Wang,BD Peyser,R Irizarry,FA Spencer,JD Boeke,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Analysis of genetic interactions on a genome-wide scale in budding yeast: diploid-based synthetic lethality analysis by microarray. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Methods in molecular biology (Clifton, N.J.)

VOLUME: 416

Page Numbers: 221-48

Journal Abbreviation: Methods Mol. Biol.

ISSN: 1064-3745

DAY: 21

MONTH: 02

YEAR: 2007

Analysis of genetic interactions on a genome-wide scale in budding yeast: diploid-based synthetic lethality analysis by microarray. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9214969

Analysis of genetic interactions on a genome-wide scale in budding yeast: diploid-based synthetic lethality analysis by microarray. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Analysis of genetic interactions on a genome-wide scale in budding yeast: diploid-based synthetic lethality analysis by microarray. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Analysis of genetic interactions on a genome-wide scale in budding yeast: diploid-based synthetic lethality analysis by microarray.

AFFILIATION:

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Methods Mol Biol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Analysis of genetic interactions on a genome-wide scale in budding yeast: diploid-based synthetic lethality analysis by microarray Related Publications

• A comprehensive synthetic genetic interaction network governing yeast histone acetylation and deacetylation.
• A descent into the nuage: the maelstrom of transposon control.
• Active retrotransposition by a synthetic L1 element in mice.
• Analysis of genetic interactions on a genome-wide scale in budding yeast: diploid-based synthetic lethality analysis by microarray.
• Analysis of genetic interactions on a genome-wide scale in budding yeast: diploid-based synthetic lethality analysis by microarray.
• Conditional activation of a single-copy L1 transgene in mice by Cre.
• DNA repair. A little help for my ends.
• Domesticated DNA transposon proteins mediate retrotransposon control.
• From the shards of a shattered genome, diversity.
• Great exaptations.
• Histone h3 k56 hyperacetylation perturbs replisomes and causes DNA damage.
• Hotspots for unselected Ty1 transposition events on yeast chromosome III are near tRNA genes and LTR sequences.
• Nucleocytosolic acetyl-coenzyme a synthetase is required for histone acetylation and global transcription.
• Probing nucleosome function: a highly versatile library of synthetic histone h3 and h4 mutants.
• Response to Dr Stephen Cooper's 'On the use of metaphor to understand, explain, or rationalize redundant genes in yeast'.
• The incredible shrinking world of DNA microarrays.
• The sympathetic endformation, its synaptology, the interstitial cells, the periterminal network, and its bearing on the neurone theory, discussion and critique.
• Transposon insertion site profiling chip (TIP-chip).
• [Advisory report from the Health Council of the Netherlands to include Human papillomavirus vaccination in the national immunisation programme for the prevention ofcervical cancer]
• [Experiences with the Grassi test.]
• [Not Available.]
• dSLAM analysis of genome-wide genetic interactions in Saccharomyces cerevisiae.