Analysis of gene structures and promoter activities of the chipmunk alpha(1)-antitrypsin-like genes.

Analysis of gene structures and promoter activities of the chipmunk alpha(1)-antitrypsin-like genes. Research Abstract Details 

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Analysis of gene structures and promoter activities of the chipmunk alpha(1)-antitrypsin-like genes. Abstract Text:

The chipmunk hibernation-specific protein HP-55 is a component of a 140-kDa complex whose levels are drastically decreased in the blood during hibernation. It is highly homologous to alpha(1)-antitrypsin (AT). In the chipmunk, several alpha(1)-AT-like genes in addition to HP-55 (or CM55-ML) are expressed in the liver and have distinct patterns of regulation during hibernation: in hibernating chipmunks, the level of CM55-ML gene expression is greatly reduced, that of the CM55-MS gene is slightly increased, and the expression of the CM55-MM gene is hardly affected. As a first step towards understanding the hibernation-associated gene regulation of these chipmunk alpha(1)-AT-like genes, we isolated genomic clones for the CM55-ML, CM55-MM, and CM55-MS genes, and analyzed their promoter activities. These alpha(1)-AT-like genes are composed of five exons, and show a similar gene structure to that of the human alpha(1)-AT gene, suggesting that they were generated by the duplication of an ancestral alpha(1)-AT gene. Transient transfection studies using HepG2 and COS-7 cells revealed that for all three alpha(1)-AT-like genes, approximately 150-bp 5' flanking sequences were sufficient for the liver-specific promoter activity, and that the binding of HNF-1 to the promoter region could transactivate transcription. In addition, analysis of the activity of chimeric promoters composed of CM55-ML and CM55-MS gene sequences indicated that the lack of a TATA box-like sequence in the CM55-MS gene is responsible for its weak promoter activity.

Analysis of gene structures and promoter activities of the chipmunk alpha(1)-antitrypsin-like genes. Publishing Authors By Initials

For similar peptides: proteinase inhibitory proteins, secretory: alpha 1-antitrypsin research abstracts see: peptides: proteinase inhibitory proteins, secretory: alpha 1-antitrypsin research

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Analysis of gene structures and promoter activities of the chipmunk alpha(1)-antitrypsin-like genes. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Gene

VOLUME: 329

Page Numbers: 71-9

Journal Abbreviation: Gene

ISSN: 0378-1119

DAY: 31

MONTH: Mar

YEAR: 2004

Analysis of gene structures and promoter activities of the chipmunk alpha(1)-antitrypsin-like genes. Information

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LANGUAGE: eng

NlmUniqueID: 7706761

Analysis of gene structures and promoter activities of the chipmunk alpha(1)-antitrypsin-like genes. Keywords Mesh Terms:

KEYWORDS: alpha 1-Antitrypsin

MESH TERMS: genetics

Chemical & Substance for Abstract: Analysis of gene structures and promoter activities of the chipmunk alpha(1)-antitrypsin-like genes. Information

Substance Name: Luciferases

Registry Number: EC 1.13.12.-

Grant and Affiliation Information for Analysis of gene structures and promoter activities of the chipmunk alpha(1)-antitrypsin-like genes.

AFFILIATION: Department of Biosciences, School of Science, Kitasato University, 1-15-1 Kitasato, Sagamihara, Kanagawa 228-8555, Japan.

Country: Netherlands

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MEDLINETA: Gene

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ACCESSION NUMBER: AB100365

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