Analysis of free hormone fractions by an ultrafast immunoextraction/displacement immunoassay: studies using free thyroxine as a model system.

Analysis of free hormone fractions by an ultrafast immunoextraction/displacement immunoassay: studies using free thyroxine as a model system. Research Abstract Details 

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Analysis of free hormone fractions by an ultrafast immunoextraction/displacement immunoassay: studies using free thyroxine as a model system. Abstract Text:

William Clarke,John E Schiel,Annette Moser,David S Hage,

A system was developed for measuring the noncomplexed or free fraction of a hormone in serum based on the combined use of ultrafast immunoextraction with a chromatographic displacement immunoassay. This approach was tested using L-thyroxine as a model analyte. Items considered in the development of this technique included the choice of immunoassay format and the selection of conditions for removal of thyroxine's free fraction from samples without significant interference from its protein-bound fraction. The final method had an effective extraction time of 90 ms and allowed the amount of free thyroxine to be determined within 30 s after sample injection. The limit of detection was 6 pM (S/N = 3) for a 100-microL sample, and the linear response extended up to at least 100 pM. This technique gave good correlation versus reference methods when used for the determination of free thyroxine in serum samples. Advantages of this method included its speed and its ability to analyze a sample with no pretreatment other than standard filtration. The same approach could be adapted for other hormones or drugs by using appropriate antibodies and labeled analogues for such agents.

Analysis of free hormone fractions by an ultrafast immunoextraction/displacement immunoassay: studies using free thyroxine as a model system. Publishing Authors By Initials

W Clarke,JE Schiel,A Moser,DS Hage,

For similar hormones, hormone substitutes, and hormone antagonists: hormones: thyroid hormones: thyroxine research abstracts see: hormones, hormone substitutes, and hormone antagonists: hormones: thyroid hormones: thyroxine research

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Analysis of free hormone fractions by an ultrafast immunoextraction/displacement immunoassay: studies using free thyroxine as a model system. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Analytical chemistry

VOLUME: 77

Page Numbers: 1859-66

Journal Abbreviation: Anal. Chem.

ISSN: 0003-2700

DAY: 15

MONTH: Mar

YEAR: 2005

Analysis of free hormone fractions by an ultrafast immunoextraction/displacement immunoassay: studies using free thyroxine as a model system. Information

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LANGUAGE: eng

NlmUniqueID: 370536

Analysis of free hormone fractions by an ultrafast immunoextraction/displacement immunoassay: studies using free thyroxine as a model system. Keywords Mesh Terms:

KEYWORDS: Thyroxine

MESH TERMS: blood

Chemical & Substance for Abstract: Analysis of free hormone fractions by an ultrafast immunoextraction/displacement immunoassay: studies using free thyroxine as a model system. Information

Substance Name: Thyroxine

Registry Number: 7488-70-2

Grant and Affiliation Information for Analysis of free hormone fractions by an ultrafast immunoextraction/displacement immunoassay: studies using free thyroxine as a model system.

AFFILIATION: Department of Chemistry, University of Nebraska, Lincoln, Nebraska 68588-0304, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: R01 GM44931

ACRONYM: GM

MEDLINETA: Anal Chem

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