Analysis of Escherichia coli ribosomal proteins by an improved two dimensional gel electrophoresis. I. Detection of four new proteins.

Analysis of Escherichia coli ribosomal proteins by an improved two dimensional gel electrophoresis. I. Detection of four new proteins. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Analysis of Escherichia coli ribosomal proteins by an improved two dimensional gel electrophoresis. I. Detection of four new proteins. Abstract Text:

A Wada,

Kaltschmidt and Wittmann's two dimensional gel electrophoresis was improved in the following points. Preruns using radical scavengers were carried out to eliminate free radicals remaining in gels. Gelation of sample solutions was not performed to avoid immobilization of proteins in the sample gels. Instead, for preparing sample gels, prior to the first dimension (1-D) electrophoresis, another electrophoresis was performed to charge proteins into gel pieces polymerized previously. Proteins migrated together with charged reductants to avoid formation of artificial disulfide bridges during migration. The second dimension (2-D) electrophoresis was carried out at a more acidic pH, 3.6, to get better separation of very small and basic proteins. With these modifications, quantitative yield and reproducibility became better, and many faint spots disappeared not only at the high molecular weight side but also in the region containing primary spots of ribosomal proteins. The proportionality of the migration distance to the logarithm of molecular weight was also increased. On the improved 2-D electrophoretogram of Escherichia coli ribosomal proteins, four new spots, called protein A, B, C, and D, were found in the basic region. Proteins A, B, and C belong to 50S subunits but D to 30S. Their molecular weights were determined electrophoretically as 6,400, 4,900, 8,200, and 5,900, respectively. Their copy numbers in crude ribosomes were estimated to be 0.6, 0.4, 0.3, and 0.1, respectively, by using 14C-labeling.

Analysis of Escherichia coli ribosomal proteins by an improved two dimensional gel electrophoresis. I. Detection of four new proteins. Publishing Authors By Initials

A Wada,

For similar proteins: ribosomal proteins research abstracts see: proteins: ribosomal proteins research

PUBMED ID PMID:

MEDLINE DATE:

Analysis of Escherichia coli ribosomal proteins by an improved two dimensional gel electrophoresis. I. Detection of four new proteins. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 100

Page Numbers: 1583-94

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Dec

YEAR: 1986

Analysis of Escherichia coli ribosomal proteins by an improved two dimensional gel electrophoresis. I. Detection of four new proteins. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Analysis of Escherichia coli ribosomal proteins by an improved two dimensional gel electrophoresis. I. Detection of four new proteins. Keywords Mesh Terms:

KEYWORDS: Ribosomal Proteins

MESH TERMS: analysis

Chemical & Substance for Abstract: Analysis of Escherichia coli ribosomal proteins by an improved two dimensional gel electrophoresis. I. Detection of four new proteins. Information

Substance Name: Ribosomal Proteins

Registry Number: 0

Grant and Affiliation Information for Analysis of Escherichia coli ribosomal proteins by an improved two dimensional gel electrophoresis. I. Detection of four new proteins.

AFFILIATION:

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Analysis of Escherichia coli ribosomal proteins by an improved two dimensional gel electrophoresis I Detection of four new proteins Related Publications

• A comparative study of the plasma membrane permeabilization and fluidization induced by antipsychotic drugs in the rat brain.
• A method for accurate estimation of stimulating sites based on cerebral cortex structure in transcranial magnetic stimulation.
• Alpha 1-adrenoceptor agonists protect against stress-induced death of neural progenitor cells.
• Application to robot control using brain function measurement by near-infrared spectroscopy.
• Arabidopsis TRANSPARENT TESTA GLABRA2 is directly regulated by R2R3 MYB transcription factors and is involved in regulation of GLABRA2 transcription in epidermal differentiation.
• Association between trait anxiety and endothelial function observed in elderly males but not in young males.
• Can Human Isochrony be Explained by a Computational Theory?
• Chloroplast photorelocation movement mediated by phototropin family proteins in green plants.
• Collectrin, a homologue of ACE2, its transcriptional control and functional perspectives.
• Dielectric dispersion of alternating copolymers of maleic acid.
• Dietary Carnosine And Anserine Inhibits High-intensity Exercise-induced Decrease In Rat Sarcoplasmic Reticulum Ca2+ -handling: 2073: Board #143 May 31 3:30 PM 5:00 PM.
• Differential expression of a subunit isoforms of the vacuolar-type proton pump ATPase in mouse endocrine tissues.
• Effect of beta-lactotensin on acute stress and fear memory.
• Effects of heat stress on filamentous actin and prestin of outer hair cells in mice.
• Estimation of force motor command for NIRS-based BMI.
• Functional analysis of the epidermal-specific MYB genes CAPRICE and WEREWOLF in Arabidopsis.
• Glycogen synthase kinase-3beta: homologous regulation of cell surface insulin receptor level via controlling insulin receptor mRNA stability in adrenal chromaffin cells.
• Identification of novel chemical inhibitors for ubiquitin C-terminal hydrolase-L3 by virtual screening.
• Life style-related diseases of the digestive system: from molecular mechanisms to therapeutic strategies: preface.
• Movement time planning in human movement with via-points.
• New method for simultaneous measurement of frequency spectra of complex dielectric constants of polyelectrolyte solutions.
• Preface.
• Remediation of Pb-contaminated soils by washing with hydrochloric acid and subsequent immobilization with calcite and allophanic soil.
• Sodium D(2) resonance radiation in single-pass sum-frequency generation with actively mode-locked Nd:YAG lasers.
• The association between anger-related personality trait and cardiac autonomic response abnormalities in elderly subjects.
• Topological and chronological features of the impairment of glucose metabolism induced by 1-methyl-4-phenylpyridinium ion (MPP(+)) in rat brain slices.
• [Anti-tuberculosis chemotherapy]
• [Diastolic heart failure]
• [New direction of medical malpractice dispute resolution]
• [Teaching surveillance to workers' health in nursing courses]