Analysis of an antibody pharmaceutical, tocilizumab, by capillary electrophoresis using a carboxylated capillary.

Analysis of an antibody pharmaceutical, tocilizumab, by capillary electrophoresis using a carboxylated capillary. Research Abstract Details 

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Analysis of an antibody pharmaceutical, tocilizumab, by capillary electrophoresis using a carboxylated capillary. Abstract Text:

Atsushi Taga,Soichiro Kita,Kaori Nishiura,Tomonori Hayashi,Mitsuhiro Kinoshita,Atsushi Sato,Kentaro Suzuki,Shuji Kodama,Kazuaki Kakehi,

Antibody pharmaceuticals are becoming more and more prevalent due to their excellent effectiveness in clinical medications, and are expected to allow tailor-made medical treatment for rheumatic diseases, immunosuppression in cardiac transplantation, and cancer. Antibody-type pharmaceuticals of immunoglobulin G (IgG) commonly have N-glycosylated carbohydrate chains attached to heavy chains. The carbohydrate chains play important roles in the effectiveness of antibodies. Therefore evaluation of a glycosylated species is important in the first step of quality control of antibody pharmaceuticals. In the present work, we examined capillary electrophoresis with a newly developed, chemically modified capillary, the inner surface of which is modified with carboxyl groups, for evaluation of IgG molecular species which have carbohydrate chains; tocilizumab was used as a model. The analytical system developed in the present study is useful for determining the content of non-glycosylated peptides. In the analysis of tocilizumab, the ratio of non-glycosylated peptide was estimated to be 1.23% with a relative standard deviation of 3.05%. The method affords high reproducibility with simple operation, and analysis can be completed within 6 min.

Analysis of an antibody pharmaceutical, tocilizumab, by capillary electrophoresis using a carboxylated capillary. Publishing Authors By Initials

A Taga,S Kita,K Nishiura,T Hayashi,M Kinoshita,A Sato,K Suzuki,S Kodama,K Kakehi,

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Analysis of an antibody pharmaceutical, tocilizumab, by capillary electrophoresis using a carboxylated capillary. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of separation science

VOLUME: 31

Page Numbers: 853-8

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ISSN: 1615-9306

DAY: 24

MONTH: Mar

YEAR: 2008

Analysis of an antibody pharmaceutical, tocilizumab, by capillary electrophoresis using a carboxylated capillary. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Analysis of an antibody pharmaceutical, tocilizumab, by capillary electrophoresis using a carboxylated capillary.

AFFILIATION: School of Pharmacy, Kinki University, Higashi-osaka, Japan. Fax: +81-6-6721-2353.

Country: Germany

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MEDLINETA: J Sep Sci

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