An optimised methodology for the neurobehavioural assessment in rodents.

An optimised methodology for the neurobehavioural assessment in rodents. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

An optimised methodology for the neurobehavioural assessment in rodents. Abstract Text:

Elena Moscardo,Anne Maurin,Roberto Dorigatti,Pascal Champeroux,Serge Richard,

INTRODUCTION: The most widely used test to identify undesired effects of drugs on the central and the peripheral nervous system is the neurobehavioural observation battery adapted from that first described by Irwin in mice. As a neurobehavioural assessment is based on observations; thus, all factors involved need to be controlled and standardised to make the data collected objective, reproducible, reliable and predictive of safety liabilities. METHODS: An observation battery comprising 58 signs with assigned full details of numerical scores was defined, and a standard design with associated recording, presenting and analysing data system was established. Validation studies were conducted with chlorpromazine, amphetamine, diazepam or clonidine given orally to rats or mice, in order to assess if this methodology could clearly differentiate the profile of effects produced by these compounds. The analysis of data from 80 control rats allowed for the assessment of the normal behaviour in order to characterise the inter-individual, daytime-related variability and the habituation of animals to the procedure. RESULTS: The reference compounds induced their typical and expected transient effects on neurobehaviour, observed both in the home cage and open-arena, and on body temperature. In particular, amphetamine induced a stimulation of the nervous system activities and marked hyperthermia. Chlorpromazine, diazepam and clonidine induced depressive, anxiolytic or sedative effects associated with hypothermia. The analysis of data collected in control animals allowed for the identification of 6 signs which scored differently from the assigned normality at the first handling occasion due to the characteristic fear reactions to the unknown, and 9 signs at 8 h post-dose due to the animal's habituation to experimental conditions and handling. DISCUSSION: The neurobehavioural changes expected by reference compounds administration were detected. These results confirm that by using this methodology the normal behaviour of the rat and the mouse, the daytime-related variability and the habituation of animals can be characterised, allowing a refined, reliable and reproducible neurobehavioural assessment of test substances in rodents.

An optimised methodology for the neurobehavioural assessment in rodents. Publishing Authors By Initials

E Moscardo,A Maurin,R Dorigatti,P Champeroux,S Richard,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE: 2007 Sep-Oct

An optimised methodology for the neurobehavioural assessment in rodents. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of pharmacological and toxicological metho

VOLUME: 56

Page Numbers: 239-55

Journal Abbreviation:

ISSN: 1056-8719

DAY: 24

MONTH: 05

YEAR: 2007

An optimised methodology for the neurobehavioural assessment in rodents. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9206091

An optimised methodology for the neurobehavioural assessment in rodents. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: An optimised methodology for the neurobehavioural assessment in rodents. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for An optimised methodology for the neurobehavioural assessment in rodents.

AFFILIATION: Safety Pharmacology, Safety Assessment Department, GlaxoSmithKline R&D Centre, Via Fleming 4, VERONA (37135), Italy. elena.2.moscardo@gsk.com

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Pharmacol Toxicol Methods

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

An optimised methodology for the neurobehavioural assessment in rodents Related Publications

• A novel GJA 1 mutation in oculo-dento-digital dysplasia with curly hair and hyperkeratosis.
• A simple method to determine kinetic deuterium isotope effects provides evidence that proton transfer to carbon proceeds over and not through the reaction barrier.
• A transition state analog for phosphate diester cleavage catalyzed by a small enzyme-like metal ion complex.
• An optimised methodology for the neurobehavioural assessment in rodents.
• Claisen-type addition of glycine to a pyridoxal iminium ion in water.
• Coming out to care: caregivers of gay and lesbian seniors in Canada.
• Covalent catalysis by pyridoxal: evaluation of the effect of the cofactor on the carbon acidity of glycine.
• Dissection of a genetic locus influencing renal function in the rat and its concordance with kidney disease loci on human chromosome 1q21.
• Energy expenditure: a critical determinant of energy balance with key hypothalamic controls.
• Enzymatic catalysis of proton transfer at carbon: activation of triosephosphate isomerase by phosphite dianion.
• Foamy Virus Vectors Expressing Anti-HIV Transgenes Efficiently Block HIV-1 Replication.
• Identification in the mould Hypocrea jecorina of a gene encoding an NADP(+): d-xylose dehydrogenase.
• Improvement of VO2max by cardiac output and oxygen extraction adaptation during intermittent versus continuous endurance training.
• Inhibition of humic substances mediated photooxygenation of furfuryl alcohol by 2,4,6-trimethylphenol. Evidence for reactivity of the phenol with humic triplet excited states.
• Malignant proliferating pilar tumors arising in KID syndrome: a report of two patients.
• Phenotypic Correction of alpha-Sarcoglycan Deficiency by Intra-arterial Injection of a Muscle-specific Serotype 1 rAAV Vector.
• Practical identifiability of photophysical parameters in photodynamic therapy.
• Product deuterium isotope effect for orotidine 5'-monophosphate decarboxylase: evidence for the existence of a short-lived carbanion intermediate.
• Rational design of transition-state analogues as potent enzyme inhibitors with therapeutic applications.
• Some major mycotoxins and their mycotoxicoses-An overview.
• Targeting the RNA-binding protein Sam68 as a treatment for cancer?
• The motivational interview.
• The motivational interview: In practice.
• Topical tacrolimus treatment for chronic dermatitis of the ear.
• Use of local area facilities for involvement in physical activity in Canada: insights for developing environmental and policy interventions.
• When does an intermediate become a transition state? Degenerate isomerization without competing racemization during solvolysis of (S)-1-(3-nitrophenyl)ethyl tosylate.
• Winning the battle for standardization. The U.S. Army Medical Department examines the EMR to develop a standardized process for medication reconciliation documentation.
• [Carbon dioxide laser therapy for anogenital warts in children.]
• [The lessons of Cleopatra or reflections on conter-transfer and borderlines.]
• [The prevalence of bilateral malignant uveal melanoma]