An oligonucleotide microarray resource for transcriptional profiling of Bradyrhizobium japonicum.

An oligonucleotide microarray resource for transcriptional profiling of Bradyrhizobium japonicum. Research Abstract Details 

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An oligonucleotide microarray resource for transcriptional profiling of Bradyrhizobium japonicum. Abstract Text:

Woo-Suk Chang,William L Franck,Eddie Cytryn,Sooyoung Jeong,Trupti Joshi,David W Emerich,Michael J Sadowsky,Dong Xu,Gary Stacey,Woo-Suk Chang,William L Franck,Eddie Cytryn,Sooyoung Jeong,Trupti Joshi,David W Emerich,Michael J Sadowsky,Dong Xu,Gary Stacey,Woo-Suk Chang,William L Franck,Eddie Cytryn,Sooyoung Jeong,Trupti Joshi,David W Emerich,Michael J Sadowsky,Dong Xu,Gary Stacey,

A DNA microarray, comprising 70-mer oligonucleotides, representing 8,453 open reading frames (ORFs), was constructed based on the Bradyrhizobium japonicum strain USDA110 genomic sequence. New annotation predicted 199 additional genes, which were added to the microarray and were shown to be transcribed. These arrays were used to profile transcription in cells under a variety of conditions, including growth in minimal versus rich medium, osmotic stress, and free-living cells versus bacteroids. Increased expression was seen for genes involved in translation, motility, and cell envelope synthesis in rich medium whereas expression increased in minimal medium for genes involved in vitamin biosynthesis and stress responses. Treatment with 50 mM NaCl activated stress-inducible genes but repressed genes involved in chemotaxis and motility. Strikingly, no known transport systems for accumulation of compatible solutes or osmoprotectants were induced in response to osmotic stress. A number of nif, fix, and hup genes, but not all, were upregulated in bacteroids. The B. japonicum type III secretion system, known to be important in early nodulation, was downregulated in bacteroids. The availability of a reliable, low-cost B. japonicum microarray provides a useful tool for functional genomic studies of one of the most agriculturally important bacteria.

An oligonucleotide microarray resource for transcriptional profiling of Bradyrhizobium japonicum. Publishing Authors By Initials

WS Chang,WL Franck,E Cytryn,S Jeong,T Joshi,DW Emerich,MJ Sadowsky,D Xu,G Stacey,WS Chang,WL Franck,E Cytryn,S Jeong,T Joshi,DW Emerich,MJ Sadowsky,D Xu,G Stacey,WS Chang,WL Franck,E Cytryn,S Jeong,T Joshi,DW Emerich,MJ Sadowsky,D Xu,G Stacey,

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An oligonucleotide microarray resource for transcriptional profiling of Bradyrhizobium japonicum. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Molecular plant-microbe interactions : MPMI

VOLUME: 20

Page Numbers: 1298-307

Journal Abbreviation: Mol. Plant Microbe Interact.

ISSN: 0894-0282

DAY: 8

MONTH: Oct

YEAR: 2007

An oligonucleotide microarray resource for transcriptional profiling of Bradyrhizobium japonicum. Information

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LANGUAGE: eng

NlmUniqueID: 9107902

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Grant and Affiliation Information for An oligonucleotide microarray resource for transcriptional profiling of Bradyrhizobium japonicum.

AFFILIATION: National Center for Soybean Biotechnology, University of Missouri, Columbia 65211, USA. changwo@missouri.edu

Country: United States

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MEDLINETA: Mol Plant Microbe Interact

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ACCESSION NUMBER: GSE8042

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