An in vivo cytotoxicity threshold for influenza A virus-specific effector and memory CD8(+) T cells.

An in vivo cytotoxicity threshold for influenza A virus-specific effector and memory CD8(+) T cells. Research Abstract Details 

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An in vivo cytotoxicity threshold for influenza A virus-specific effector and memory CD8(+) T cells. Abstract Text:

John Stambas,Peter C Doherty,Stephen J Turner,

Influenza A virus infection of C57BL/6 (B6) mice is characterized by prominent CD8(+) T cell responses to H2D(b) complexed with peptides from the viral nucleoprotein (NP(366), ASNENMETM) and acid polymerase (PA(224), SSLENFRAYV). An in vivo cytotoxicity assay that depends on the adoptive transfer of peptide-pulsed, syngeneic targets was used in this study to quantitate the cytotoxic potential of D(b)NP(366)- and D(b)PA(224)-specific acute and memory CD8(+) T cells following primary or secondary virus challenge. Both T cell populations displayed equivalent levels of in vivo effector function when comparable numbers were transferred into naive B6 hosts. Cytotoxic activity following primary infection clearly correlated with the frequency of tetramer-stained CD8(+) T cells. This relationship looked, however, to be less direct following secondary exposure, partly because the numbers of CD8(+)D(b)NP(366)(+) T cells were greatly in excess. However, calculating the in vivo E:T ratios indicated that in vivo lysis, like many other biological functions, is threshold dependent. Furthermore, the capacity to eliminate peptide-pulsed targets was independent of the differentiation state (i.e., primary or secondary effectors) and was comparable for the two T cell specificities that were analyzed. These experiments provide insights that may be of value for adoptive immunotherapy, where careful consideration of both the activation state and the number of effector cells is required.

An in vivo cytotoxicity threshold for influenza A virus-specific effector and memory CD8(+) T cells. Publishing Authors By Initials

J Stambas,PC Doherty,SJ Turner,

For similar proteins: viral proteins: viral structural proteins: nucleocapsid proteins: viral core proteins research abstracts see: proteins: viral proteins: viral structural proteins: nucleocapsid proteins: viral core proteins research

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An in vivo cytotoxicity threshold for influenza A virus-specific effector and memory CD8(+) T cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of immunology (Baltimore, Md. : 1950)

VOLUME: 178

Page Numbers: 1285-92

Journal Abbreviation: J. Immunol.

ISSN: 0022-1767

DAY: 1

MONTH: Feb

YEAR: 2007

An in vivo cytotoxicity threshold for influenza A virus-specific effector and memory CD8(+) T cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985117

An in vivo cytotoxicity threshold for influenza A virus-specific effector and memory CD8(+) T cells. Keywords Mesh Terms:

KEYWORDS: Viral Core Proteins

MESH TERMS: immunology

Chemical & Substance for Abstract: An in vivo cytotoxicity threshold for influenza A virus-specific effector and memory CD8(+) T cells. Information

Substance Name: nucleoprotein (366-374), influenza virus

Registry Number: 0

Grant and Affiliation Information for An in vivo cytotoxicity threshold for influenza A virus-specific effector and memory CD8(+) T cells.

AFFILIATION: Department of Microbiology and Immunology, University of Melbourne, Parkville, Melbourne, Australia.

Country: United States

AGENCY: United States NIAID

GRANT: AI170251

ACRONYM: AI

MEDLINETA: J Immunol

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