An in vitro selection system for TNA.

An in vitro selection system for TNA. Research Abstract Details 

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An in vitro selection system for TNA. Abstract Text:

Justin K Ichida,Keyong Zou,Allen Horhota,Biao Yu,Larry W McLaughlin,Jack W Szostak,

(3'-2')-alpha-l-Threose nucleic acid (TNA) is an unnatural polymer that possesses the rare ability to base-pair with RNA, DNA, and itself. This feature, coupled with its chemical simplicity, makes TNA of interest as a possible progenitor of RNA during the early history of life. To evaluate the functional potential of TNA, we have developed a system for the in vitro selection of TNA. We identified the Therminator DNA polymerase as a remarkably efficient DNA-dependent TNA polymerase capable of polymerizing more than 50 tNTPs. We have also developed a method of covalently linking a DNA template to the TNA strand that it encodes, thus obviating the need for a TNA-dependent DNA polymerase during cycles of selection.

An in vitro selection system for TNA. Publishing Authors By Initials

JK Ichida,K Zou,A Horhota,B Yu,LW McLaughlin,JW Szostak,

For similar carbohydrates: monosaccharides: tetroses research abstracts see: carbohydrates: monosaccharides: tetroses research

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An in vitro selection system for TNA. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of the American Chemical Society

VOLUME: 127

Page Numbers: 2802-3

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 9

MONTH: Mar

YEAR: 2005

An in vitro selection system for TNA. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7503056

An in vitro selection system for TNA. Keywords Mesh Terms:

KEYWORDS: Tetroses

MESH TERMS: chemistry

Chemical & Substance for Abstract: An in vitro selection system for TNA. Information

Substance Name: DNA-Directed DNA Polymerase

Registry Number: EC 2.7.7.7

Grant and Affiliation Information for An in vitro selection system for TNA.

AFFILIATION: Howard Hughes Medical Institute, and Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: R01 GM53936

ACRONYM: GM

MEDLINETA: J Am Chem Soc

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