An experimental study of extracellular signal-regulated kinase and its interventional treatments in hepatic fibrosis.

An experimental study of extracellular signal-regulated kinase and its interventional treatments in hepatic fibrosis. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

An experimental study of extracellular signal-regulated kinase and its interventional treatments in hepatic fibrosis. Abstract Text:

Ming-De Jiang,Shu-Mei Zheng,Hui Xu,Wei-Zheng Zeng,Yong Zhang,Hao-Ping Sun,Yun-Xia Wang,Jian-Ping Qin,Xiao-Ling Wu,Ming-De Jiang,Shu-Mei Zheng,Hui Xu,Wei-Zheng Zeng,Yong Zhang,Hao-Ping Sun,Yun-Xia Wang,Jian-Ping Qin,Xiao-Ling Wu,

BACKGROUND: The pathogenesis of hepatic fibrosis and cirrhosis is still not fully understood. The extracellular signal-regulated kinase (ERK) pathway is involved in the regulation of cell proliferation and differentiation. The aim of this study was to investigate the effects of PD98059, a specific inhibitor of ERK, on the cell cycle, cell proliferation, secretion of type I collagen and expression of cyclin D1 mRNA, CDK4 mRNA and transforming growth factor-beta1 (TGF-beta1) mRNA in rat hepatic stellate cells (HSCs) stimulated by acetaldehyde. METHODS: Rat HSCs stimulated by acetaldehyde were incubated with PD98059 at different concentrations. The cell cycle was analysed by flow cytometry. Cell proliferation was assessed by the methyl thiazolyl tetrazolium colorimetric assay. The mRNA expression of cyclin D1, CDK4 and TGF-beta1 was examined using the reverse transcriptase-polymerase chain reaction. Type I collagen in the culture medium was detected by enzyme-linked immunosorbent assay. RESULTS: 20, 50 and 100 mumol/L PD98059 significantly inhibited the proliferation and provoked a G0/G1-phase arrest of acetaldehyde-induced HSCs in a dose-dependent manner. The secretion of type I collagen and the expression of cyclin D1, CDK4 and TGF-beta1 mRNA in acetaldehyde-induced HSCs were markedly inhibited by 50 and 100 mumol/L PD98059, respectively. CONCLUSIONS: The ERK pathway regulates the cell proliferation, secretion of type I collagen and the expression of TGF-beta1 mRNA in rat HSCs stimulated by acetaldehyde, which is likely related to its regulative effect on the cell cycle.

An experimental study of extracellular signal-regulated kinase and its interventional treatments in hepatic fibrosis. Publishing Authors By Initials

MD Jiang,SM Zheng,H Xu,WZ Zeng,Y Zhang,HP Sun,YX Wang,JP Qin,XL Wu,MD Jiang,SM Zheng,H Xu,WZ Zeng,Y Zhang,HP Sun,YX Wang,JP Qin,XL Wu,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

An experimental study of extracellular signal-regulated kinase and its interventional treatments in hepatic fibrosis. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Hepatobiliary & pancreatic diseases international

VOLUME: 7

Page Numbers: 51-7

Journal Abbreviation: HBPD INT

ISSN: 1499-3872

DAY: 31

MONTH: Feb

YEAR: 2008

An experimental study of extracellular signal-regulated kinase and its interventional treatments in hepatic fibrosis. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101151457

An experimental study of extracellular signal-regulated kinase and its interventional treatments in hepatic fibrosis. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: An experimental study of extracellular signal-regulated kinase and its interventional treatments in hepatic fibrosis. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for An experimental study of extracellular signal-regulated kinase and its interventional treatments in hepatic fibrosis.

AFFILIATION: Department of Gastroenterology, General Hospital of Chengdu Military Command, Chengdu 610083, China. zhengsm@163.com.

Country: China

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Hepatobiliary Pancreat Dis Int

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

An experimental study of extracellular signal-regulated kinase and its interventional treatments in hepatic fibrosis Related Publications

• Characterization of human mucin 5B gene expression in airway epithelium and the genomic clone of the amino-terminal and 5'-flanking region.
• Clinical analysis of 4 cases of juvenile granulosa cell tumor of the ovary.
• Differential regulation of airway mucin gene expression and mucin secretion by extracellular nucleotide triphosphates.
• Effects of As(2)O(3) on BCR-ABL protein level and signal transduction in CML cells.
• Etiological analysis on the ocular fungal infection.
• Expression of mPer1 and mPer2, two mammalian clock genes, in murine bone marrow.
• Monocyte chemoattractant protein-1 mRNA in human intracranial aneurysm walls.
• Roles of kappa opioid receptors in cardioprotection against ischemia: the signaling mechanisms.
• Significance of HER-2/neu expression in ovarian epithelial tumours.
• Stimulation of airway mucin gene expression by interleukin (IL)-17 through IL-6 paracrine/autocrine loop.
• Study on some mechanism of leucite microcrystallization to reinforce dental glass ceramics.
• The clinical study of lateral orbitotomy.
• The expression, characterization and roles of macrophage colony-stimulating factor receptor in human leukemia cell lines.
• Thermal hyperalgesic effects induced by intracerebroventricular injection of interleukin-1beta in rats.
• [Arsenic exposure of residents in areas near Shimen arsenic mine]
• [Clinical study of treatment with continuous renal replacement therapy on severe hepatitis with hepatic encephalopathy]
• [Content of metals in different diameter airborne particles and effect on micronuclei formation in human lymphocytes]
• [Detection of monosomy 7 or 7q- in cases of myelodysplastic syndrome]
• [Determination of triptolide in tripterygium preparations by gradient high performance liquid chromatography]
• [Discussion on the concept problem of the specific retention volume in gas chromatography]
• [Effect of recombinant human bone morphogenetic protein-2 on the expression of osteopontin in human periodontal ligament cells]
• [Liver sinusoidal endothelial cell damage during normothermic ischemia and reperfusion of rat]
• [Oral combination chemotherapy of 5'-deoxy-5-fluorouridine (furtulon) and cyclophosphamide for advanced breast cancer]
• [Pathological features of atrial isomerism and its diagnosis by computed tomography]
• [Preliminary study on the molecular mechanism of K562 cell apoptosis induced by As2S2]
• [Relationship between apolipoprotein E and apolipoprotein B polymorphisms in youths with coronary heart disease]
• [Screening and identification of egg mimotope of Schistosoma japonicum]
• [Studies on immunoprotection in mice after immunization with Schistosoma japonicum 22.6 kDa recombinant protein]
• [Study of acute physiology and chronic health evaluation III and multiple organ dysfunction syndrome score in evaluating severity of intensive care units patient]
• [Study on hepatic-protective effect of "yinzhihuang" granula]