An evolvable oestrogen receptor activity sensor: development of a modular system for integrating multiple genes into the yeast genome.

An evolvable oestrogen receptor activity sensor: development of a modular system for integrating multiple genes into the yeast genome. Research Abstract Details 

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An evolvable oestrogen receptor activity sensor: development of a modular system for integrating multiple genes into the yeast genome. Abstract Text:

Jennifer E Fox,Jamie T Bridgham,Toine F H Bovee,Joseph W Thornton,

To study a gene interaction network, we developed a gene-targeting strategy that allows efficient and stable genomic integration of multiple genetic constructs at distinct target loci in the yeast genome. This gene-targeting strategy uses a modular plasmid with a recyclable selectable marker and a multiple cloning site into which the gene of interest is cloned, flanked by two long regions of homology to the target genomic locus that are generated using adaptamer primers. We used this strategy to integrate into a single yeast strain components of the oestrogen receptor (ER) signalling network, comprising the human ERalpha and three reporter genes driven by oestrogen response elements (EREs). The engineered strain contains multiple reporters of ligand-dependent receptor signalling, providing sensitive, reproducible, rapid, low-cost quantitative assays of ERalpha activity in order to screen potential receptor agonists. Further, because two of the ERE-driven reporter genes are required for growth in deficient media, the strain's growth rate-and therefore its fitness-depends on ligand-induced ERalpha activity. This evolvable oestrogen receptor activity sensor (EERAS) can therefore provide the foundation of a long-term experimental evolution strategy to elucidate ER structure-function relations and ligand-receptor evolution.

An evolvable oestrogen receptor activity sensor: development of a modular system for integrating multiple genes into the yeast genome. Publishing Authors By Initials

JE Fox,JT Bridgham,TF Bovee,JW Thornton,

For similar genetic processes: recombination, genetic: transformation, genetic research abstracts see: genetic processes: recombination, genetic: transformation, genetic research

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An evolvable oestrogen receptor activity sensor: development of a modular system for integrating multiple genes into the yeast genome. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Yeast (Chichester, England)

VOLUME: 24

Page Numbers: 379-90

Journal Abbreviation:

ISSN: 0749-503X

DAY: 3

MONTH: May

YEAR: 2007

An evolvable oestrogen receptor activity sensor: development of a modular system for integrating multiple genes into the yeast genome. Information

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LANGUAGE: eng

NlmUniqueID: 8607637

An evolvable oestrogen receptor activity sensor: development of a modular system for integrating multiple genes into the yeast genome. Keywords Mesh Terms:

KEYWORDS: Transformation, Genetic

MESH TERMS: physiology

Chemical & Substance for Abstract: An evolvable oestrogen receptor activity sensor: development of a modular system for integrating multiple genes into the yeast genome. Information

Substance Name: Estradiol

Registry Number: 50-28-2

Grant and Affiliation Information for An evolvable oestrogen receptor activity sensor: development of a modular system for integrating multiple genes into the yeast genome.

AFFILIATION: Center for Ecology and Evolutionary Biology, University of Oregon, Eugene, OR 97403, USA.

Country: England

AGENCY: United States NIGMS

GRANT: R21GM70792

ACRONYM: GM

MEDLINETA: Yeast

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