An evolutionary and molecular analysis of Bmp2 expression.

An evolutionary and molecular analysis of Bmp2 expression. Research Abstract Details 

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An evolutionary and molecular analysis of Bmp2 expression. Abstract Text:

Kevin L Abrams,Junwang Xu,Celine Nativelle-Serpentini,Shabnam Dabirshahsahebi,Melissa B Rogers,

The coding regions of many metazoan genes are highly similar. For example, homologs to the key developmental factor bone morphogenetic protein (BMP) 2 have been cloned by sequence identity from arthropods, mollusks, cnidarians, and nematodes. Wide conservation of protein sequences suggests that differential gene expression explains many of the vast morphological differences between species. To test the hypothesis that the regulatory mechanisms controlling this evolutionarily ancient and critical gene are conserved, we compared sequences flanking Bmp2 genes of several species. We identified numerous conserved noncoding sequences including some retained because the fish lineage separated 450 million years ago. We tested the function of some of these sequences in the F9 cell model system of Bmp2 expression. We demonstrated that both mouse and primate Bmp2 promoters drive a reporter gene in an expression pattern resembling that of the endogenous transcript in F9 cells. A conserved Sp1 site contributes to the retinoic acid responsiveness of the Bmp2 promoter, which lacks a classical retinoic acid response element. We have also discovered a sequence downstream of the stop codon whose conservation between humans, rodents, deer, chickens, frogs, and fish is striking. A fragment containing this region influences reporter gene expression in F9 cells. The conserved region contains elements that may mediate the half-life of the Bmp2 transcript. Together, our molecular and evolutionary analysis has identified new regulatory elements controlling Bmp2 expression.

An evolutionary and molecular analysis of Bmp2 expression. Publishing Authors By Initials

KL Abrams,J Xu,C Nativelle-Serpentini,S Dabirshahsahebi,MB Rogers,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

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An evolutionary and molecular analysis of Bmp2 expression. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: The Journal of biological chemistry

VOLUME: 279

Page Numbers: 15916-28

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 2

MONTH: 02

YEAR: 2004

An evolutionary and molecular analysis of Bmp2 expression. Information

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LANGUAGE: eng

NlmUniqueID: 2985121

An evolutionary and molecular analysis of Bmp2 expression. Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: genetics

Chemical & Substance for Abstract: An evolutionary and molecular analysis of Bmp2 expression. Information

Substance Name: bone morphogenetic protein 2

Registry Number: 0

Grant and Affiliation Information for An evolutionary and molecular analysis of Bmp2 expression.

AFFILIATION: Department of Biology, University of South Florida, Tampa, Florida 33620, USA.

Country: United States

AGENCY: United States NICHD

GRANT: HD31117

ACRONYM: HD

MEDLINETA: J Biol Chem

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