An ethanol-tolerant recombinant Escherichia coli expressing Zymomonas mobilis pdc and adhB genes for enhanced ethanol production from xylose.

An ethanol-tolerant recombinant Escherichia coli expressing Zymomonas mobilis pdc and adhB genes for enhanced ethanol production from xylose. Research Abstract Details 

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An ethanol-tolerant recombinant Escherichia coli expressing Zymomonas mobilis pdc and adhB genes for enhanced ethanol production from xylose. Abstract Text:

Zhanchao Wang,Ming Chen,Yuquan Xu,Shuying Li,Wei Lu,Shuzhen Ping,Wei Zhang,Min Lin,

An ethanol-tolerant mutant, ET1, was isolated by an enrichment method from Escherichia coli JM109. Strains JM109 and ET1 were transformed with expression vector pZY507bc containing Zymomonas mobilis alcohol dehydrogenase II (adhB) and pyruvate decarboxylase (pdc) genes, resulting in an ethanol-sensitive recombinant strain JMbc and an ethanol-tolerant recombinant strain, ET1bc. Alcohol dehydrogenase and pyruvate decarboxylase activities were 24 and 32% lower, respectively, in JMbc than in ET1bc. ET1bc fermented 10% (w/v) xylose to give 39.4 g ethanol/l (77%, theoretical yield), a 1.3-fold increase compared with the ethanol-sensitive strain JMbc.

An ethanol-tolerant recombinant Escherichia coli expressing Zymomonas mobilis pdc and adhB genes for enhanced ethanol production from xylose. Publishing Authors By Initials

Z Wang,M Chen,Y Xu,S Li,W Lu,S Ping,W Zhang,M Lin,

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An ethanol-tolerant recombinant Escherichia coli expressing Zymomonas mobilis pdc and adhB genes for enhanced ethanol production from xylose. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biotechnology letters

VOLUME: 30

Page Numbers: 657-63

Journal Abbreviation: Biotechnol. Lett.

ISSN: 0141-5492

DAY: 22

MONTH: 11

YEAR: 2007

An ethanol-tolerant recombinant Escherichia coli expressing Zymomonas mobilis pdc and adhB genes for enhanced ethanol production from xylose. Information

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LANGUAGE: eng

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Grant and Affiliation Information for An ethanol-tolerant recombinant Escherichia coli expressing Zymomonas mobilis pdc and adhB genes for enhanced ethanol production from xylose.

AFFILIATION: College of Biological Sciences, China Agricultural University, Beijing, 100094, P.R. China.

Country: Netherlands

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MEDLINETA: Biotechnol Lett

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