An EST survey of the sugarcane transcriptome.

An EST survey of the sugarcane transcriptome. Research Abstract Details 

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An EST survey of the sugarcane transcriptome. Abstract Text:

H-M Ma,S Schulze,S Lee,M Yang,E Mirkov,J Irvine,P Moore,A Paterson,

Its large genome and high polyploidy makes sugarcane (Saccharum spp.) a singularly challenging crop to study and improve using genetic approaches. To provide large numbers of functionally characterized candidate genes that might be tested for direct association (rather than distant linkage) with economically important traits, we sequenced the 5' ends of 9,216 clones from three cDNA libraries (apex, leaf and mature internode), representing 3,401 non-redundant sequences. About 57% of these sequences could be assigned a tentative function based on statistically significant similarity to previously characterized proteins or DNA sequences. Another 28% corresponded to previously identified, but uncharacterized, sequences. Some of the remaining unidentified sequences were predicted to be genes which could potentially be new to plants or unique to sugarcane. Comparisons of the sugarcane ESTs to a large sorghum EST database revealed similar compositions of expressed genes between some different tissues. Comparison to a detailed Arabidopsis protein database showed some highly conserved sequences, which might be useful DNA markers for pan-angiosperm comparative mapping. These EST sequences provide a foundation for many new studies to accelerate isolation of agronomically important genes from the cumbersome sugarcane genome.

An EST survey of the sugarcane transcriptome. Publishing Authors By Initials

HM Ma,S Schulze,S Lee,M Yang,E Mirkov,J Irvine,P Moore,A Paterson,

For similar plants: plant families and groups: angiosperms: poaceae: saccharum research abstracts see: plants: plant families and groups: angiosperms: poaceae: saccharum research

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An EST survey of the sugarcane transcriptome. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: TAG. Theoretical and applied genetics. Theoretisch

VOLUME: 108

Page Numbers: 851-63

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ISSN: 0040-5752

DAY: 29

MONTH: 11

YEAR: 2003

An EST survey of the sugarcane transcriptome. Information

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LANGUAGE: eng

NlmUniqueID: 145600

An EST survey of the sugarcane transcriptome. Keywords Mesh Terms:

KEYWORDS: Saccharum

MESH TERMS: genetics

Chemical & Substance for Abstract: An EST survey of the sugarcane transcriptome. Information

Substance Name: RNA, Messenger

Registry Number: 0

Grant and Affiliation Information for An EST survey of the sugarcane transcriptome.

AFFILIATION: Plant Genome Mapping Laboratory, University of Georgia, 111 Riverbend Rd., Athens, GA 30602, USA.

Country: Germany

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MEDLINETA: Theor Appl Genet

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