An enhancer activates the pig lactase phlorizin hydrolase promoter in intestinal cells.

An enhancer activates the pig lactase phlorizin hydrolase promoter in intestinal cells. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

An enhancer activates the pig lactase phlorizin hydrolase promoter in intestinal cells. Abstract Text:

Lactase phlorizin hydrolase is a small intestinal-specific brush border protein commonly used as a specific marker of differentiated enterocytes. A number of transcription factors involved in the enterocyte-specific expression of lactase phlorizin hydrolase have been identified. An upstream regulatory region, which we have named the "LPH enhancer", located at position -894 to -798 in the porcine lactase phlorizin hydrolase gene, is necessary for high differentiation-dependent LPH expression in intestinal cells. The LPH enhancer was studied by mutation analysis, transfection experiments and electrophoretical mobility shift assays. The LPH enhancer is active in intestinal cells (Caco-2) and not in non-intestinal cells (HeLa). The LPH enhancer is only able to enhance expression when it is located in front of an intestinal-specific promoter such as the lactase phlorizin hydrolase promoter or the sucrase-isomaltase promoter. In front of an SV40-derived promoter the LPH enhancer has no stimulatory effect. In addition to the lack of promoter-promiscuity, the LPH enhancer is not a classical enhancer in the sense that it is not orientation-independent and it cannot function when located 3' of a reporter gene. The LPH enhancer contains at least three cis-elements (at -894 to -880, -880 to -875 and -833 to -814) with functional importance for the LPH enhancer activity.

An enhancer activates the pig lactase phlorizin hydrolase promoter in intestinal cells. Publishing Authors By Initials

For similar proteins: transcription factors research abstracts see: proteins: transcription factors research

PUBMED ID PMID:

MEDLINE DATE:

An enhancer activates the pig lactase phlorizin hydrolase promoter in intestinal cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Gene

VOLUME: 305

Page Numbers: 101-11

Journal Abbreviation: Gene

ISSN: 0378-1119

DAY: 13

MONTH: Feb

YEAR: 2003

An enhancer activates the pig lactase phlorizin hydrolase promoter in intestinal cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7706761

An enhancer activates the pig lactase phlorizin hydrolase promoter in intestinal cells. Keywords Mesh Terms:

KEYWORDS: Transcription Factors

MESH TERMS: physiology

Chemical & Substance for Abstract: An enhancer activates the pig lactase phlorizin hydrolase promoter in intestinal cells. Information

Substance Name: Glycosylceramidase

Registry Number: EC 3.2.1.62

Grant and Affiliation Information for An enhancer activates the pig lactase phlorizin hydrolase promoter in intestinal cells.

AFFILIATION: Department of Medical Biochemistry and Genetics, Biochemical Laboratory C, The Panum Institute, University of Copenhagen, Blegdamsvej 3, DK-2200 N Copenhagen, Denmark. troelsen@imbg.ku.dk

Country: Netherlands

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Gene

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

An enhancer activates the pig lactase phlorizin hydrolase promoter in intestinal cells Related Publications

• Autoantibody profiling to identify individuals at risk for systemic lupus erythematosus.
• Bone morphogenetic protein receptors and bone morphogenetic protein signaling are controlled by tumor necrosis factor-alpha in human bone cells.
• Changes in select redox proteins of the retinal pigment epithelium in age-related macular degeneration.
• Coffee and smoking as risk factors of twin pregnancies: the Danish National Birth Cohort.
• Effects of calcium ion-implantation of titanium on bone cell function in vitro.
• Effects of chronic opioid exposure on guinea pig mu opioid receptor in Chinese hamster ovary cells: comparison with human and rat receptor.
• Exercise training decreases rat heart mitochondria free radical generation but does not prevent Ca2+-induced dysfunction.
• Exogenous transforming growth factor-beta(2) enhances connective tissue formation in transforming growth factor-beta(1)-deficient, healing-impaired dermal wounds in mice.
• Gene genealogies and population variation in plants.
• Increased myeloid cell responses to M-CSF and RANKL cause bone loss and inflammation in SH3BP2 "cherubism" mice.
• Iron supplement use among Danish pregnant women.
• Is maternal obesity related to semen quality in the male offspring? A pilot study.
• Ischemic stroke and n-3 fatty acids.
• Kinesin-2 controls development and patterning of the vertebrate skeleton by Hedgehog- and Gli3-dependent mechanisms.
• Lessons from genetic forms of osteoarthritis for the pathogenesis of the disease.
• Leukocytosis in acute stroke: Relation to initial stroke severity, infarct size, and outcome: The copenhagen stroke study.
• Low dose acute alcohol effects on GABA A receptor subtypes.
• Mechanical force-induced midpalatal suture remodeling in mice.
• Posterior repair and sexual function.
• Risk for cancer in patients with Bardet-Biedl syndrome and their relatives.
• Risk of early or severe pre-eclampsia related to pre-existing conditions.
• Risks for skin and other cancers up to 25 years after burn injuries.
• Shedding of a soluble form of BMP receptor-IB controls bone cell responses to BMP.
• Short-Term Oral Diazepam Treatment during Pregnancy : A Population-Based Teratological Case-Control Study.
• The high-pressure phase of zincite.
• The role of backscattering in SHG tissue imaging.
• The role of trehalose dimycolate (cord factor) on morphology of virulent M. tuberculosis in vitro.
• Toxoplasmosis lymphadenitis presenting as a parotid mass: a report of 2 cases.
• Up-regulation of bone morphogenetic protein receptor IB by growth factors enhances BMP-2-induced human bone cell functions.
• When is hot not so hot? Fever reduces brain inhibition.