An electrochemiluminescence-based competitive displacement immunoassay for the type-2 brevetoxins in oyster extracts.

An electrochemiluminescence-based competitive displacement immunoassay for the type-2 brevetoxins in oyster extracts. Research Abstract Details 

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An electrochemiluminescence-based competitive displacement immunoassay for the type-2 brevetoxins in oyster extracts. Abstract Text:

A new competitive electrochemiluminescence-based immunoassay for the type-2 brevetoxins in oyster extracts was developed. The assay was verified by spiking known amounts of PbTx-3 into 80% methanol extracts of Gulf Coast oysters. We also provide preliminary data demonstrating that 100% acetone extracts, aqueous homogenates, and the clinical matrixes urine and serum can also be analyzed without significant matrix interferences. The assay offers the advantages of speed ( 2 h analysis time); simplicity (only 2 additions, one incubation period, and no wash steps before analysis); low limit of quantitation (conservatively, 50 pg/mL = 1 ng/g tissue equivalents); and a stable, nonradioactive label. Due to the variety of brevetoxin metabolites present and the lack of certified reference standards for liquid chromatography-mass spectrometry confirmation, a true validation of brevetoxins in shellfish extracts is not possible at this time. However, our assay correlated well with another brevetoxin immunoassay currently in use in the United States. We believe this assay could be useful as a regulatory screening tool and could support pharmacokinetic studies in animals and clinical evaluation of neurotoxic shellfish poisoning victims.

An electrochemiluminescence-based competitive displacement immunoassay for the type-2 brevetoxins in oyster extracts. Publishing Authors By Initials

For similar complex mixtures: tissue extracts research abstracts see: complex mixtures: tissue extracts research

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An electrochemiluminescence-based competitive displacement immunoassay for the type-2 brevetoxins in oyster extracts. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of AOAC International

VOLUME: 90

Page Numbers: 173-8

Journal Abbreviation: J AOAC Int

ISSN: 1060-3271

DAY: 17

MONTH: 03

YEAR: 2008

An electrochemiluminescence-based competitive displacement immunoassay for the type-2 brevetoxins in oyster extracts. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9215446

An electrochemiluminescence-based competitive displacement immunoassay for the type-2 brevetoxins in oyster extracts. Keywords Mesh Terms:

KEYWORDS: Tissue Extracts

MESH TERMS: analysis

Chemical & Substance for Abstract: An electrochemiluminescence-based competitive displacement immunoassay for the type-2 brevetoxins in oyster extracts. Information

Substance Name: brevetoxin

Registry Number: 98225-48-0

Grant and Affiliation Information for An electrochemiluminescence-based competitive displacement immunoassay for the type-2 brevetoxins in oyster extracts.

AFFILIATION: United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702-5011, USA. mark.poli@det.amedd.army.mil

Country: United States

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MEDLINETA: J AOAC Int

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