An easy cell-free protein synthesis system dependent on the addition of crude Escherichia coli tRNA.

An easy cell-free protein synthesis system dependent on the addition of crude Escherichia coli tRNA. Research Abstract Details 

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An easy cell-free protein synthesis system dependent on the addition of crude Escherichia coli tRNA. Abstract Text:

T Kanda,K Takai,S Yokoyama,H Takaku,

The protein-synthesizing S30 extract of Escherichia coli contains tRNA, which limits its applications in cell-free protein synthesis. Here, we show that at least Arg- and Ser-acceptor activities can be removed from a standard S30 extract by treatment with an immobilized RNase A resin. This RNase-treated extract exhibits no protein synthesis activity, but regains it when supplied with crude E. coli tRNA and a small amount of human placental RNase inhibitor. The protein synthesis is dependent on the addition of tRNA in the presence of the RNase inhibitor. Chloramphenicol acetyltransferase was synthesized with this system and found to be active.

An easy cell-free protein synthesis system dependent on the addition of crude Escherichia coli tRNA. Publishing Authors By Initials

T Kanda,K Takai,S Yokoyama,H Takaku,

For similar proteins: ribosomal proteins research abstracts see: proteins: ribosomal proteins research

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An easy cell-free protein synthesis system dependent on the addition of crude Escherichia coli tRNA. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 127

Page Numbers: 37-41

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jan

YEAR: 2000

An easy cell-free protein synthesis system dependent on the addition of crude Escherichia coli tRNA. Information

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LANGUAGE: eng

NlmUniqueID: 376600

An easy cell-free protein synthesis system dependent on the addition of crude Escherichia coli tRNA. Keywords Mesh Terms:

KEYWORDS: Ribosomal Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: An easy cell-free protein synthesis system dependent on the addition of crude Escherichia coli tRNA. Information

Substance Name: Ribonuclease, Pancreatic

Registry Number: EC 3.1.27.5

Grant and Affiliation Information for An easy cell-free protein synthesis system dependent on the addition of crude Escherichia coli tRNA.

AFFILIATION: Department of Industrial Chemistry, Chiba Institute of Technology, Tsudanuma, Narashino, Chiba 275-0016, Japan.

Country: JAPAN

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MEDLINETA: J Biochem

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