An avidin-based assay for histone debiotinylase activity in human cell nuclei.

An avidin-based assay for histone debiotinylase activity in human cell nuclei. Research Abstract Details 

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An avidin-based assay for histone debiotinylase activity in human cell nuclei. Abstract Text:

Yap Ching Chew,Gautam Sarath,Janos Zempleni,

The covalent binding of biotin to histones participates in heterochromatin formation, cell cycle progression and cellular response to DNA breaks. Biotinylation of histones appears to be a reversible process, but the identity of enzymes that remove biotin marks is largely unknown. Our long-term goal is to identify histone debiotinylases in human cells. Here we developed an avidin-based plate assay to quantify histone debiotinylase activities in nuclear extracts. This assay is an essential first step in purifying and identifying histone debiotinylases from human cells. Using this assay, we demonstrated that debiotinylation of histones depends on temperature and pH, consistent with enzyme catalysis. Experiments with purified histones, proteases and protease inhibitors provide evidence that removal of biotin marks from histones is mediated by debiotinylases rather than by proteases. Activities of histone debiotinylases varied among human tissues: colon=lung>placenta=liver>lymphoid cells. This assay proved useful in monitoring activities of putative histone debiotinylases during their partial purification from cells. Collectively, this assay is a useful tool for investigating histone debiotinylases in human tissues.

An avidin-based assay for histone debiotinylase activity in human cell nuclei. Publishing Authors By Initials

YC Chew,G Sarath,J Zempleni,

For similar chemical and pharmacologic phenomena: kinetics research abstracts see: chemical and pharmacologic phenomena: kinetics research

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An avidin-based assay for histone debiotinylase activity in human cell nuclei. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: The Journal of nutritional biochemistry

VOLUME: 18

Page Numbers: 475-81

Journal Abbreviation: J. Nutr. Biochem.

ISSN: 0955-2863

DAY: 6

MONTH: 12

YEAR: 2006

An avidin-based assay for histone debiotinylase activity in human cell nuclei. Information

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LANGUAGE: eng

NlmUniqueID: 9010081

An avidin-based assay for histone debiotinylase activity in human cell nuclei. Keywords Mesh Terms:

KEYWORDS: Kinetics

MESH TERMS: metabolism

Chemical & Substance for Abstract: An avidin-based assay for histone debiotinylase activity in human cell nuclei. Information

Substance Name: Biotinidase

Registry Number: EC 3.5.1.12

Grant and Affiliation Information for An avidin-based assay for histone debiotinylase activity in human cell nuclei.

AFFILIATION: Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE 68583-0806, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: R01 DK063945-03

ACRONYM: DK

MEDLINETA: J Nutr Biochem

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