An automated fluorescent single strand conformation polymorphism technique for high throughput mutation screening.

An automated fluorescent single strand conformation polymorphism technique for high throughput mutation screening. Research Abstract Details 

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An automated fluorescent single strand conformation polymorphism technique for high throughput mutation screening. Abstract Text:

OBJECTIVE: To develop a high throughput mutational detection method by multiple fluorescence-labeled polymerase chain reaction (PCR) products. METHODS: A total of 27 known mutations including 22 substitutions, 3 insertions (1, 2 and 7 bp) and 2 deletions (1 and 2 bp) in the hepatocyte nuclear factor (HNF)-4 alpha, glucokinase and HNF-1 alpha genes were tested. During nested PCR, amplified fragments were labeled with three fluorescent dyes. PCR products were visualized with an ABI-377 fluorescence sequencer using 5% glycerol or 10% sucrose in non-denaturing gel conditions. RESULTS: Twenty-five of 27 variants (93%) could be detected by combining 5% glycerol and 10% sucrose gel matrix conditions. Twenty-two of 27 (82%) and 18 of 27 (67%) variants were identified using 5% glycerol and 10% sucrose conditions, respectively. CONCLUSION: This fluorescence-based PCR single strand conformation polymorphism technique represents a simple, non-hazardous, time-saving and sensitive method for high throughput mutation detection.

An automated fluorescent single strand conformation polymorphism technique for high throughput mutation screening. Publishing Authors By Initials

For similar proteins: transcription factors research abstracts see: proteins: transcription factors research

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An automated fluorescent single strand conformation polymorphism technique for high throughput mutation screening. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Chinese medical journal

VOLUME: 114

Page Numbers: 1147-50

Journal Abbreviation: Chin. Med. J.

ISSN: 0366-6999

DAY: 14

MONTH: Nov

YEAR: 2001

An automated fluorescent single strand conformation polymorphism technique for high throughput mutation screening. Information

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LANGUAGE: eng

NlmUniqueID: 7513795

An automated fluorescent single strand conformation polymorphism technique for high throughput mutation screening. Keywords Mesh Terms:

KEYWORDS: Transcription Factors

MESH TERMS: genetics

Chemical & Substance for Abstract: An automated fluorescent single strand conformation polymorphism technique for high throughput mutation screening. Information

Substance Name: Glucokinase

Registry Number: EC 2.7.1.2

Grant and Affiliation Information for An automated fluorescent single strand conformation polymorphism technique for high throughput mutation screening.

AFFILIATION: Department of Endocrinology, First Affiliated Hospital of Sun Yet-San University of Medical Sciences, Guangzhou 510080, China. gzwengjp@public.guangzhou.gd.cn

Country: China

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MEDLINETA: Chin Med J (Engl)

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