An assay for methotrexate and its metabolites in serum and urine by ion-pair high-performance liquid chromatography.

An assay for methotrexate and its metabolites in serum and urine by ion-pair high-performance liquid chromatography. Research Abstract Details 

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An assay for methotrexate and its metabolites in serum and urine by ion-pair high-performance liquid chromatography. Abstract Text:

Y Y Farid,I D Watson,M J Stewart,

A high-performance liquid chromatographic assay for methotrexate and its metabolites, 7-hydroxy-methotrexate, 4-amino-4-deoxy-N(10)-methyl-pteroic acid and 7-hydroxy-4-amino-4-deoxy-N(10)-methyl-pteroic acid in the range 10 mu/l to 50 mg/l (2.2 x 10(-8) to 1.1. x 10(-4)M) has been developed using l-tryptophyl-l-glutamic acid as internal standard. Extraction was performed using an anion exchange resin (Dowex 1-X2) with subsequent ion-pair chromatography of the appropriate eluent fraction. The method has been found to be sensitive and precise for the analysis of both serum and urine, and may also be used for the quantitation of polyglutamyl metabolites.

An assay for methotrexate and its metabolites in serum and urine by ion-pair high-performance liquid chromatography. Publishing Authors By Initials

YY Farid,ID Watson,MJ Stewart,

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An assay for methotrexate and its metabolites in serum and urine by ion-pair high-performance liquid chromatography. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of pharmaceutical and biomedical analysis

VOLUME: 1

Page Numbers: 55-63

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ISSN: 0731-7085

DAY: 26

MONTH: 07

YEAR: 1983

An assay for methotrexate and its metabolites in serum and urine by ion-pair high-performance liquid chromatography. Information

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LANGUAGE: eng

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Grant and Affiliation Information for An assay for methotrexate and its metabolites in serum and urine by ion-pair high-performance liquid chromatography.

AFFILIATION: Department of Pathological Biochemistry, Royal Infirmary, Glasgow G4 OSF, Scotland, UK.

Country: England

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MEDLINETA: J Pharm Biomed Anal

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