An Arabidopsis MLH1 mutant exhibits reproductive defects and reveals a dual role for this gene in mitotic recombination.

An Arabidopsis MLH1 mutant exhibits reproductive defects and reveals a dual role for this gene in mitotic recombination. Research Abstract Details 

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An Arabidopsis MLH1 mutant exhibits reproductive defects and reveals a dual role for this gene in mitotic recombination. Abstract Text:

Eric Dion,Liangliang Li,Martine Jean, Belzile,

The eukaryotic DNA mismatch repair (MMR) system contributes to maintaining genome integrity and DNA sequence fidelity in at least two important ways: by correcting errors arising during DNA replication, and also by preventing recombination events between divergent sequences. This study aimed to investigate the role of one key MMR gene in recombination. We obtained a mutant line in which the AtMLH1 gene has been disrupted by the insertion of a T-DNA within the coding region. Transcript analysis indicated that no full-length transcript was produced in mutant plants. The loss of a functional AtMLH1 gene led to a significant reduction in fertility in both homozygotes and heterozygotes, and we observed a strong bias against transmission of the mutant allele. To investigate the role of AtMLH1 in mitotic recombination, the mutant was crossed to a series of recombination reporter lines. A strong decrease (72%) in the frequency of homologous recombination was observed in the mutant. However, the decline in recombination due to homeology was less severe in the Atmlh1 mutant than in a wild-type control. These data demonstrate a dual role for AtMLH1 in recombination: it is both required for recombination and acts to limit recombination between diverged sequences.

An Arabidopsis MLH1 mutant exhibits reproductive defects and reveals a dual role for this gene in mitotic recombination. Publishing Authors By Initials

E Dion,L Li,M Jean,F Belzile,

For similar genetic processes: recombination, genetic research abstracts see: genetic processes: recombination, genetic research

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An Arabidopsis MLH1 mutant exhibits reproductive defects and reveals a dual role for this gene in mitotic recombination. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Plant journal : for cell and molecular biology

VOLUME: 51

Page Numbers: 431-40

Journal Abbreviation: Plant J.

ISSN: 0960-7412

DAY: 8

MONTH: 06

YEAR: 2007

An Arabidopsis MLH1 mutant exhibits reproductive defects and reveals a dual role for this gene in mitotic recombination. Information

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LANGUAGE: eng

NlmUniqueID: 9207397

An Arabidopsis MLH1 mutant exhibits reproductive defects and reveals a dual role for this gene in mitotic recombination. Keywords Mesh Terms:

KEYWORDS: Recombination, Genetic

MESH TERMS: genetics

Chemical & Substance for Abstract: An Arabidopsis MLH1 mutant exhibits reproductive defects and reveals a dual role for this gene in mitotic recombination. Information

Substance Name: MLH1 protein, Arabidopsis

Registry Number: EC 3.6.1.-

Grant and Affiliation Information for An Arabidopsis MLH1 mutant exhibits reproductive defects and reveals a dual role for this gene in mitotic recombination.

AFFILIATION: Département de phytologie, 1243 Pavillon C.-E. Marchand, Université Laval, Québec, QC, G1K 7P4, Canada.

Country: England

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MEDLINETA: Plant J

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